Dual mechanism β-amino acid polymers promoting cell adhesion.
Dual mechanism β-amino acid polymers promoting cell adhesion.
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双机制β-氨基酸聚合物促进细胞粘附
DOI：
10.1038/s41467-020-20858-x
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发表时间：
2021-01-25
影响因子：
16.6
通讯作者：
Liu R
中科院分区：
文献类型：
--
作者：
Chen Q;Zhang D;Zhang W;Zhang H;Zou J;Chen M;Li J;Yuan Y;Liu R
细胞粘附对培养平台和植入物的功能有很大的影响。细胞粘附蛋白和多肽在促进细胞粘附方面已被广泛应用了几十年，然而，它们的应用受到易酶降解、难以大规模制备和价格昂贵的困扰。为了开发新一代细胞粘附材料，我们模拟了RGD和KRSR肽的细胞粘附功能和机制，设计了在蛋白质水解后稳定且易于大规模制备的阳离子疏水两亲性β-氨基酸聚合物。以成骨前细胞为模型，通过遵循与统计共聚物的序列和手性无关的双重机制，优选出的聚合物能强烈促进细胞粘附。我们的策略为设计下一代细胞粘附材料开辟了道路，并可能指导未来的研究和应用。像RGD这样的细胞粘附肽在生物医学应用中很重要，但存在蛋白水解、加工和成本问题。在这里，作者报告了阳离子疏水两亲性β-氨基酸聚合物的发展，其功能是细胞粘附基序，但耐蛋白水解。
Cell adhesion has tremendous impact on the function of culture platforms and implants. Cell-adhesive proteins and peptides have been extensively used for decades to promote cell adhesion, however, their application suffers from their easy enzymatic degradation, difficulty in large-scale preparation and expensiveness. To develop the next-generation cell-adhesive materials, we mimic the cell adhesion functions and mechanisms of RGD and KRSR peptides and design cell-adhesive cationic-hydrophobic amphiphilic β-amino acid polymers that are stable upon proteolysis and easily prepared in large scale at low cost. The optimal polymer strongly promotes cell adhesion, using preosteoblast cell as a model, by following dual mechanisms that are independent of sequence and chirality of the statistic copolymer. Our strategy opens avenues in designing the next-generation cell-adhesive materials and may guide future studies and applications. Cell adhesion peptides like RGD are important to biomedical applications but suffer from proteolysis as well as processing and cost issues. Here, the authors report on the development of cationic-hydrophobic amphiphilic β-amino acid polymers which function as cell adhesion motifs but are resistant to proteolysis.
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影响因子：
16.1
作者：
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通讯作者：
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影响因子：
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通讯作者：
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作者：
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影响因子：
5.8
作者：
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通讯作者：
Chen, Zetao