The transcriptome of the early life history stages of the California Sea Hare Aplysia californica.
The transcriptome of the early life history stages of the California Sea Hare Aplysia californica.
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DOI：
10.1016/j.cbd.2010.03.003
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发表时间：
2010-06
影响因子：
3
通讯作者：
Walsh, P. J.
中科院分区：
文献类型：
--
作者：
Fiedler, T. J.;Hudder, A.;Mckay, S. J.;Shivkumar, S.;Capo, T. R.;Schmale, M. C.;Walsh, P. J.
加州无尾两栖动物是一种海洋后鳃类软体动物，因为它拥有相对较少的大尺寸神经元，所以在神经生物学中被用作学习和行为的细胞分析的模式生物。软体动物是第二大动物门，但详细的遗传和基因组信息只是最近才开始积累。因此，发育和比较进化生物学以及生物医学研究将受益于更多的信息，DNA序列的Aaplasia。因此，我们构建了一系列的单向cDNA文库，从不同的生活阶段的Aesthesia。这些包括来自卵、面盘幼虫、变态和幼年阶段的整个生物体以及成年神经组织供参考。随机挑选单个克隆，并进行高通量、单次通过序列分析以产生7971个序列。其中，有5507质量过滤的EST，聚类成1988 unigenes，这是注释和存入GenBank。大量的EST（497）与现有的拟南芥EST不匹配，因此可能是拟南芥的新序列。这些新序列的GO和KEGG分析表明，大量参与蛋白质结合和翻译，与发育中的主要生物合成作用和阶段特异性蛋白质亚型的存在一致。
Aplysia californica is a marine opisthobranch mollusc used as a model organism in neurobiology for cellular analyses of learning and behavior because it possesses a comparatively small number of neurons of large size. The mollusca comprise the second largest animal phylum, yet detailed genetic and genomic information is only recently beginning to accrue. Thus developmental and comparative evolutionary biology as well as biomedical research would benefit from additional information on DNA sequences of Aplysia. Therefore, we have constructed a series of unidirectional cDNA libraries from different life stages of Aplysia. These include whole organisms from the egg, veliger, metamorphic, and juvenile stages as well as adult neural tissue for reference. Individual clones were randomly picked, and high-throughput, single pass sequence analysis was performed to generate 7971 sequences. Of these, there were 5507 quality-filtered ESTs that clustered into 1988 unigenes, which are annotated and deposited into GenBank. A significant number (497) of ESTs did not match existing Aplysia ESTs and are thus potentially novel sequences for Aplysia. GO and KEGG analyses of these novel sequences indicated that a large number were involved in protein binding and translation, consistent with the predominant biosynthetic role in development and the presence of stage-specific protein isoforms.
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影响因子：
14.9
作者：
UniProt Consortium
通讯作者：
UniProt Consortium
Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
10.1016/j.cub.2009.01.052
发表时间：
2009-03-10
期刊：
Current biology : CB
影响因子：
--
作者：
Martin BL;Kimelman D
通讯作者：
Kimelman D
影响因子：
4.1
作者：
Knudsen, B;Kohn, AB;Moroz, LL
通讯作者：
Moroz, LL