Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment.
Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment.
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DOI：
10.1016/j.joen.2009.10.030
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发表时间：
2010-02
影响因子：
4.2
通讯作者：
Kapila, Yvonne Lorraine
中科院分区：
文献类型：
--
作者：
Garcia Paula-Silva, Francisco Wanderley;Bezerra da Silva, Lea Assed;Kapila, Yvonne Lorraine
关键词：
探讨基质金属蛋白酶（MMPs）在根尖牙炎及根管治疗后根尖周愈合期的表达。根尖牙周炎引起的狗牙齿和根管治疗进行了一次访问或使用额外的氢氧化钙根管敷料。治疗后180天检查炎症的存在，并对组织进行染色以检测细菌。细菌状态与组织组织程度相关，为了进一步研究参与这一过程的分子，我们对组织进行了MMP-1、MMP-2、MMP-8和MMP-9染色。数据分析采用单因素方差分析后采用Tukey检验或Kruskal-Wallis后采用Dunn检验。根尖牙周炎的牙齿进行根管治疗进行了一次访问呈现强烈的炎症细胞浸润。根尖周围组织极度混乱，这与细菌的存在有关。MMP表达明显升高，与未治疗的根尖牙周炎相似。相比之下，根尖牙周炎的牙齿在使用氢氧化钙进行根管治疗时，炎症细胞浸润较低。这一组具有中等组织的结缔组织，较低的细菌患病率和较低数量的mmp阳性细胞，类似于接受治疗的健康牙齿。与单次治疗不同，用氢氧化钙根管敷料治疗的牙齿表现出较低的细菌污染百分比，较低的MMP表达和更有组织的ECM。这表明氢氧化钙可能在组织修复过程中有益。
To investigate the expression of matrix metalloproteinases (MMPs) in apical periodontitis and during the periapical healing phase following root canal treatment. Apical periodontitis was induced in dog teeth and root canal treatment was performed in a single visit or using an additional calcium hydroxide root canal dressing. One hundred and eighty days following treatment the presence of inflammation was examined and tissues were stained to detect bacteria. Bacterial status was correlated to the degree of tissue organization, and to further investigate molecules involved in this process, tissues were stained for MMP-1, MMP-2, MMP-8, and MMP-9. Data were analyzed using one-way ANOVA followed by Tukey test or Kruskal-Wallis followed by Dunn. Teeth with apical periodontitis that had root canal therapy performed in a single visit presented an intense inflammatory cell infiltrate. Periapical tissue was extremely disorganized, and this was correlated with the presence of bacteria. Higher MMP expression was evident, similar to teeth with untreated apical periodontitis. In contrast, teeth with apical periodontitis submitted to root canal treatment using calcium hydroxide presented a lower inflammatory cell infiltrate. This group had a moderately organized connective tissue, a lower prevalence of bacteria, and a lower number of MMP-positive cells, similar to healthy teeth submitted to treatment. Teeth treated with calcium hydroxide root canal dressing exhibited a lower percentage of bacterial contamination, a lower MMP expression, and a more organized ECM, unlike those treated in a single visit. This suggests that calcium hydroxide may be beneficial in tissue repair processes.
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影响因子：
3.3
作者：
Hong, CY;Lin, SK;Wang, JS
通讯作者：
Wang, JS
DOI：
10.1111/j.1749-6632.1994.tb24719.x
发表时间：
1994-01-01
期刊：
INHIBITION OF MATRIX METALLOPROTEINASES: THERAPEUTIC POTENTIAL
影响因子：
--
作者：
KRANE, SM
通讯作者：
KRANE, SM
影响因子：
5
作者：
Liapatas, S;Nakou, M;Rontogianni, D
通讯作者：
Rontogianni, D
影响因子：
4.2
作者：
Huang, Fu-Mei;Yang, Shun-Fa;Chang, Yu-Chao
通讯作者：
Chang, Yu-Chao
影响因子：
4.2
作者：
Garcia de Paula-Silva, Francisco Wanderley;D'Silva, Nishaj J.;Bezerra da Silva, Lea Assed;Kapila, Yvonne Lorraine
通讯作者：
Kapila, Yvonne Lorraine