Transformation of sporozoites into early exoerythrocytic malaria parasites does not require host cells.
Transformation of sporozoites into early exoerythrocytic malaria parasites does not require host cells.
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DOI：
10.1084/jem.20022100
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发表时间：
2003-04-21
期刊：
影响因子：
--
通讯作者：
Kappe SH
中科院分区：
文献类型：
--
作者：
Kaiser K;Camargo N;Kappe SH
感染包括人类在内的哺乳动物的疟疾寄生虫物种在开始感染红细胞导致疟疾疾病之前，必须首先以红细胞外形式（EEF）驻留在肝宿主细胞中。尽管肝脏阶段对疟疾免疫的重要性，但对其生物学和抗原组成知之甚少。在这里，我们表明，子孢子，寄生虫的传播阶段，驻留在蚊子载体唾液腺，可以转化为早期EEF没有细胞内驻留在宿主肝细胞。转化的形态序列和EEF中蛋白质的表达似乎与在宿主细胞内发育的寄生虫没有区别。转化取决于温度升高到37°C和血清。我们的研究结果表明，居住在宿主肝细胞或特定的宿主细胞衍生的因子是没有必要带来深刻的形态和生化变化的寄生虫发生后，从载体到哺乳动物宿主的传输。
Malaria parasite species that infect mammals, including humans, must first take up residence in hepatic host cells as exoerythrocytic forms (EEF) before initiating infection of red blood cells that leads to malaria disease. Despite the importance of hepatic stages for immunity against malaria, little is known about their biology and antigenic composition. Here, we show that sporozoites, the parasites' transmission stage that resides in the mosquito vector salivary glands, can transform into early EEF without intracellular residence in host hepatocytes. The morphological sequence of transformation and the expression of proteins in the EEF appear indistinguishable from parasites that develop within host cells. Transformation depends on temperature elevation to 37°C and serum. Our findings demonstrate that residence in a host hepatocyte or specific host cell–derived factors are not necessary to bring about the profound morphological and biochemical changes of the parasite that occur after its transmission from vector to mammalian host.
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DOI：
10.4269/ajtmh.1983.32.682
发表时间：
1983-01-01
影响因子：
3.3
作者：
HOLLINGDALE, MR;LELAND, P;SCHWARTZ, AL
通讯作者：
SCHWARTZ, AL
影响因子：
3.6
作者：
MEIS, JFGM;VERHAVE, JP;MEUWISSEN, JHET
通讯作者：
MEUWISSEN, JHET
DOI：
10.1073/pnas.92.9.4066
发表时间：
1995-04-25
影响因子：
11.1
作者：
SCHELLER, LF;AZAD, AF
通讯作者：
AZAD, AF
影响因子：
3.4
作者：
Natarajan, R;Thathy, V;Vernick, KD
通讯作者：
Vernick, KD
影响因子：
64.8
作者：
Fang, J;McCutchan, TF
通讯作者：
McCutchan, TF