The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture.
The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture.
复制标题
DOI：
10.1093/rb/rbab050
复制
发表时间：
2021-10
影响因子：
6.7
通讯作者：
Weng J
中科院分区：
文献类型：
--
作者：
Shi F;Xiao D;Zhang C;Zhi W;Liu Y;Weng J
以往的研究证明，动态培养可以促进营养物质的运输，并对三维支架内的细胞施加机械刺激，从而促进干细胞向成骨表型分化。然而，在动态条件下，大孔大小对干细胞成骨分化的影响尚不清楚。因此，本研究的目的是研究在静态和灌流培养条件下，羟基磷灰石(HAP)支架的大孔尺寸对骨髓间充质干细胞成骨分化的影响。体外细胞培养结果显示，与静态培养相比，灌流培养条件下的细胞增殖、碱性磷酸酶(ALP)活性、碱性磷酸酶(ALP)、I型胶原(Col-I)、骨钙素(OCN)和骨桥蛋白(OPN)的mRNA表达均增强。在灌流培养条件下，随着大孔尺寸的减小，碱性磷酸酶的活性和碱性磷酸酶、I型胶原、骨钙素和骨桥蛋白的基因表达增强，但随着大孔尺寸的进一步减小，成骨相关基因的表达和蛋白分泌减少，而随着大孔尺寸的减小，成骨相关基因的表达和蛋白分泌减少。计算流体力学分析表明，随着大孔尺寸的减小，支架内流体剪应力增大，中高速流动的分布面积增大。这些结果证实了大孔尺寸对流体刺激和细胞分化的影响，也有助于优化骨组织工程用HAP支架的大孔尺寸。
Previous studies have proved that dynamic culture could facilitate nutrients transport and apply mechanical stimulation to the cells within three-dimensional scaffolds, thus enhancing the differentiation of stem cells towards the osteogenic phenotype. However, the effects of macropore size on osteogenic differentiation of stem cells under dynamic condition are still unclear. Therefore, the objective of this study was to investigate the effects of macropore size of hydroxyapatite (HAp) scaffolds on osteogenic differentiation of bone mesenchymal stem cells under static and perfusion culture conditions. In vitro cell culture results showed that cell proliferation, alkaline phosphate (ALP) activity, mRNA expression of ALP, collagen-I (Col-I), osteocalcin (OCN) and osteopontin (OPN) were enhanced when cultured under perfusion condition in comparison to static culture. Under perfusion culture condition, the ALP activity and the gene expression of ALP, Col-I, OCN and OPN were enhanced with the macropore size decreasing from 1300 to 800 µm. However, with the further decrease in macropore size from 800 to 500 µm, the osteogenic related gene expression and protein secretion were reduced. Computational fluid dynamics analysis showed that the distribution areas of medium- and high-speed flow increased with the decrease in macropore size, accompanied by the increase of the fluid shear stress within the scaffolds. These results confirm the effects of macropore size on fluid flow stimuli and cell differentiation, and also help optimize the macropore size of HAp scaffolds for bone tissue engineering.
登录
查看更多内容
影响因子：
14
作者：
Goldstein, AS;Juarez, TM;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
6.2
作者：
Li, Yuncang;Xiong, Jianyu;Wen, Cui'e
通讯作者：
Wen, Cui'e
影响因子：
3.8
作者：
McCoy, R. J.;Jungreuthmayer, C.;O'Brien, F. J.
通讯作者：
O'Brien, F. J.
影响因子：
3
作者：
Bhaskar, Birru;Owen, Robert;Reilly, Gwendolen C.
通讯作者：
Reilly, Gwendolen C.
影响因子：
3.7
作者：
Guignandon, A;Usson, Y;Vico, L
通讯作者：
Vico, L