Adaptation of tibial structure and strength to axial compression depends on loading history in both C57BL/6 and BALB/c mice.
Adaptation of tibial structure and strength to axial compression depends on loading history in both C57BL/6 and BALB/c mice.
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DOI：
10.1007/s00223-013-9744-4
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发表时间：
2013-09
影响因子：
4.2
通讯作者：
Silva, Matthew J.
中科院分区：
文献类型：
--
作者：
Holguin, Nilsson;Brodt, Michael D.;Sanchez, Michelle E.;Kotiya, Akhilesh A.;Silva, Matthew J.
胫骨压缩可增加小鼠骨量。然而，加载方案和小鼠品系在研究之间不同，这可能导致相互矛盾的结果。我们假设，骨积累的影响更大的负荷历史比小鼠应变或动物处理。对4月龄C57 BL/6和BALB/c小鼠的右胫骨进行轴向压缩（10 N，3天/周，6周）。将左侧胫骨作为对侧对照，以计算相对变化[（加载-对照）/对照]。WashU方案应用60个周期/天，频率为2 Hz，周期之间休息插入10 s; Cornell/HSS方案应用1200个周期/天，频率为6.7 Hz，休息插入0.1 s。由于假负荷、镇静和运输不影响胫骨形态，因此未处理的小鼠作为年龄匹配的对照（AC）。两种加载方案对皮质骨都是合成代谢的，但Cornell/HSS加载引起了更快的反应，比WashU加载大13%。6周时，各负荷组皮质骨体积均大于AC组（平均值）。16%，彼此之间没有区别。任一方案加载的胫骨的极限位移和断裂能量更大，Cornell/HSS加载的极限力更大。在6周时，独立于小鼠品系，WashU方案产生最小的骨小梁，并且Cornell/HSS钛的骨小梁体积分数比AC大65%，比WashU大44%。我们的结论是，胫骨适应负荷的波形比小鼠应变或动物处理的影响，因此可能有针对性的C57 BL/6和BALB/c小鼠相似的成骨机制。
Tibial compression can increase murine bone mass. However, loading protocols and mouse strains differ between studies which may contribute to conflicting results. We hypothesized that bone accrual is influenced more by loading history than by mouse strain or animal handling. The right tibiae of 4-month C57BL/6 and BALB/c mice were subjected to axial compression (10 N, 3 days/week, 6 weeks). Left tibiae served as contralateral controls to calculate relative changes [(Loaded-Control)/Control]. The WashU protocol applied 60 cycles/day, at 2 Hz, with 10 s rest-insertion between cycles; the Cornell/HSS protocol applied 1200 cycles/day, at 6.7 Hz, with 0.1 s rest-insertion. Because sham loading, sedation and transportation did not affect tibial morphology, unhandled mice served as age-matched controls (AC). Both loading protocols were anabolic for cortical bone, but Cornell/HSS loading elicited a more rapid response that was greater than WashU loading by 13%. By 6 weeks, cortical bone volume of each loading group was greater than of AC (avg. +16%) and not different from each other. Ultimate displacement and energy-to-fracture were greater in tibiae loaded by either protocol and ultimate force was greater with Cornell/HSS loading. At 6 weeks, independent of mouse strain, the WashU protocol produced minimal trabecular bone and the trabecular bone volume fraction of Cornell/HSS tibiae was greater than of AC by 65% and of WashU by 44%. We concluded that tibial adaptation to loading was more influenced by waveform than mouse strain or animal handling and therefore may have targeted similar osteogenic mechanisms in C57BL/6 and BALB/c mice.
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影响因子：
6.2
作者：
Robling, AG;Burr, DB;Turner, CH
通讯作者：
Turner, CH
影响因子：
4.1
作者：
Lynch, Maureen E.;Main, Russell P.;Xu, Qian;Schmicker, Thomas L.;Schaffler, Mitchell B.;Wright, Timothy M.;van der Meulen, Marjolein C. H.
通讯作者：
van der Meulen, Marjolein C. H.
影响因子：
3.3
作者：
Lynch, Maureen E.;Main, Russell P.;van der Meulen, Marjolein C. H.
通讯作者：
van der Meulen, Marjolein C. H.
影响因子：
6.2
作者：
Mori, T;Okimoto, N;Nakamura, T
通讯作者：
Nakamura, T
影响因子：
4.1
作者：
Poliachik, Sandra L.;Threet, DeWayne;Gross, Ted S.
通讯作者：
Gross, Ted S.