Simultaneous tracking of 3D actin and microtubule strains in individual MLO-Y4 osteocytes under oscillatory flow.
Simultaneous tracking of 3D actin and microtubule strains in individual MLO-Y4 osteocytes under oscillatory flow.
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DOI：
10.1016/j.bbrc.2013.01.052
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发表时间：
2013-02-22
影响因子：
3.1
通讯作者：
Guo, X. Edward
中科院分区：
文献类型：
--
作者：
Baik, Andrew D.;Qiu, Jun;Hillman, Elizabeth M. C.;Dong, Cheng;Guo, X. Edward
骨细胞在体内经历复杂的流体剪切流动模式以激活机械转导通路。肌动蛋白和微管（MT）细胞骨架已被证明在骨细胞对流体剪切负荷的生化反应中起重要作用。生理相关的流体流动曲线的动态性质（即，1 Hz振荡流）阻碍了以高时空分辨率在同一细胞中同时成像和研究肌动蛋白和MT细胞骨架的能力。为了克服这些局限性，多通道准三维显微镜技术的开发，同时跟踪肌动蛋白和MT网络下稳定和振荡流。细胞在应变谱中显示出高的细胞间变异性和细胞内细胞骨架变异性。剪切Exz是主要的应变在稳定和振荡流的粘弹性蠕变和弹性振荡的形式，分别。然而，在振荡流中看到了巨大的差异。肌动蛋白菌株显示振荡应变曲线更经常比MT网络在所有的测试菌株，并有较高的峰谷应变幅度。总之，肌动蛋白网络是振荡流下骨细胞中更敏感的细胞骨架网络，并且可能在机械转导通路的激活和调节中发挥更大的作用。
Osteocytes in vivo experience complex fluid shear flow patterns to activate mechanotransduction pathways. The actin and microtubule (MT) cytoskeletons have been shown to play an important role in the osteocyte’s biochemical response to fluid shear loading. The dynamic nature of physiologically relevant fluid flow profiles (i.e., 1 Hz oscillatory flow) impedes the ability to image and study both actin and MT cytoskeletons simultaneously in the same cell with high spatiotemporal resolution. To overcome these limitations, a multi-channel quasi-3D microscopy technique was developed to track the actin and MT networks simultaneously under steady and oscillatory flow. Cells displayed high intercellular variability and intracellular cytoskeletal variability in strain profiles. Shear Exz was the predominant strain in both steady and oscillatory flows in the form of viscoelastic creep and elastic oscillations, respectively. Dramatic differences were seen in oscillatory flow, however. The actin strains displayed an oscillatory strain profile more often than the MT networks in all the strains tested and had a higher peak-to-trough strain magnitude. Taken together, the actin networks are the more responsive cytoskeletal networks in osteocytes under oscillatory flow and may play a bigger role in mechanotransduction pathway activation and regulation.
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影响因子：
11.6
作者：
Sampathkumar, Arun;Lindeboom, Jelmer J.;Persson, Staffan
通讯作者：
Persson, Staffan
影响因子：
2.4
作者：
Kwon, Ronald Y.;Jacobs, Christopher R.
通讯作者：
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影响因子：
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作者：
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影响因子：
48
作者：
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通讯作者：
Wedlich-Soldner, Roland
DOI：
10.1073/pnas.1009382107
发表时间：
2010-08-03
影响因子：
11.1
作者：
Burra, Sirisha;Nicolella, Daniel P.;Jiang, Jean X.
通讯作者：
Jiang, Jean X.