Comparative genome analysis provides insights into the evolution and adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum.
Comparative genome analysis provides insights into the evolution and adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum.
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DOI：
10.1371/journal.pone.0010224
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发表时间：
2010-04-19
期刊：
影响因子：
3.7
通讯作者：
Kamoun S
中科院分区：
文献类型：
--
作者：
Green S;Studholme DJ;Laue BE;Dorati F;Lovell H;Arnold D;Cottrell JE;Bridgett S;Blaxter M;Huitema E;Thwaites R;Sharp PM;Jackson RW;Kamoun S
一种由丁香假单胞菌引起的出血溃疡病正在威胁欧洲西北部的欧洲七叶树。人们对这种新疾病的起源和生物学知之甚少。利用7个常用标记基因的核苷酸序列，研究了最近从英国欧洲七叶树（E-Pae）出血茎溃疡病中分离的3株菌株的系统发育。仅根据这些序列，E-Pae菌株与1969年从印度七叶树叶斑中分离到的Pae型菌株（I-Pae）相同。系统发育分析还表明，Pae属于丁香假单胞菌中一个适应木质寄主的独特分支。我们从3株E-Pae菌株和1株I-Pae菌株中获得全基因组Illumina序列数据。比较基因组分析揭示了Pae的致病特异性基因组区域可能与树宿主的毒力有关，包括植物源芳香族化合物分解代谢和肠杆菌合成的基因。一些基因簇显示出了病内变异，包括编码IV型分泌、新型脂肪酸生物合成途径和蔗糖摄取途径的基因簇。四种Pae基因组的单核苷酸多态性率表明，三种E-Pae菌株彼此分化的时间比它们与I-Pae的分化时间要晚得多。三种地理位置不同的E-Pae菌株的遗传多样性非常低，这表明它们起源于最近一次传入英国的单一菌株，从而突出了外来植物致病菌传播到新的地理位置所带来的严重环境风险。Pae中的基因组区域在其他感染草本寄主的紫丁香假单胞菌病原菌中缺失，这可能代表了对树木木质部分感染的候选遗传适应。
A recently emerging bleeding canker disease, caused by Pseudomonas syringae pathovar aesculi (Pae), is threatening European horse chestnut in northwest Europe. Very little is known about the origin and biology of this new disease. We used the nucleotide sequences of seven commonly used marker genes to investigate the phylogeny of three strains isolated recently from bleeding stem cankers on European horse chestnut in Britain (E-Pae). On the basis of these sequences alone, the E-Pae strains were identical to the Pae type-strain (I-Pae), isolated from leaf spots on Indian horse chestnut in India in 1969. The phylogenetic analyses also showed that Pae belongs to a distinct clade of P. syringae pathovars adapted to woody hosts. We generated genome-wide Illumina sequence data from the three E-Pae strains and one strain of I-Pae. Comparative genomic analyses revealed pathovar-specific genomic regions in Pae potentially implicated in virulence on a tree host, including genes for the catabolism of plant-derived aromatic compounds and enterobactin synthesis. Several gene clusters displayed intra-pathovar variation, including those encoding type IV secretion, a novel fatty acid biosynthesis pathway and a sucrose uptake pathway. Rates of single nucleotide polymorphisms in the four Pae genomes indicate that the three E-Pae strains diverged from each other much more recently than they diverged from I-Pae. The very low genetic diversity among the three geographically distinct E-Pae strains suggests that they originate from a single, recent introduction into Britain, thus highlighting the serious environmental risks posed by the spread of an exotic plant pathogenic bacterium to a new geographic location. The genomic regions in Pae that are absent from other P. syringae pathovars that infect herbaceous hosts may represent candidate genetic adaptations to infection of the woody parts of the tree.
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影响因子：
64.8
作者：
Delledonne, M;Xia, YJ;Lamb, C
通讯作者：
Lamb, C
影响因子：
3.2
作者：
GUNASEKARAN, P;KARUNAKARAN, T;BARATTI, J
通讯作者：
BARATTI, J
影响因子：
2.1
作者：
Farrer, Rhys A.;Kemen, Eric;Studholme, David J.
通讯作者：
Studholme, David J.
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A
影响因子：
3.5
作者：
Guilhabert, MR;Kirkpatrick, BC
通讯作者：
Kirkpatrick, BC