Sub-populations of Spinal V3 Interneurons Form Focal Modules of Layered Pre-motor Microcircuits.
Sub-populations of Spinal V3 Interneurons Form Focal Modules of Layered Pre-motor Microcircuits.
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DOI：
10.1016/j.celrep.2018.08.095
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发表时间：
2018-10-02
期刊：
影响因子：
8.8
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Chopek JW;Nascimento F;Beato M;Brownstone RM;Zhang Y
神经回路的分层有助于将具有高空间敏感性的神经元与那些发挥整合性时间作用的神经元分离。虽然解剖层在大脑中很容易识别，但在脊髓中分层在结构上并不明显。但是，对运动行为的计算研究已经导致了脊髓分层处理的概念。它已被假定，脊髓V3中间神经元（IN）在运动中发挥多种作用，导致我们调查他们是否形成分层的微电路。使用膜片钳记录与全息谷氨酸释放相结合，我们展示了焦点，分层模块，其中腹内侧V3 INs彼此形成突触，并与腹外侧V3 INs形成突触，这反过来又与同侧运动神经元形成突触。运动神经元，反过来，提供经常性的兴奋性，兴奋性输入到V3的INs.Thus，腹侧V3 interneurons形成分层的微电路，可以发挥作用，以确保良好的时间，空间特定的运动。腹侧脊髓V3中间神经元（IN）的两个群体可以被区分。内侧（V3VMed）和外侧（V3VLat）群体在连接模式上不同。运动神经元轴突周期性地激发同侧V3 IN。展示了脊髓内的局部分层微电路。这些微回路由两个腹侧V3中间神经元亚群和同侧运动神经元组成。从内侧到外侧建立突触连接，运动神经元反复兴奋V3中间神经元亚群。
Layering of neural circuits facilitates the separation of neurons with high spatial sensitivity from those that play integrative temporal roles. Although anatomical layers are readily identifiable in the brain, layering is not structurally obvious in the spinal cord. But computational studies of motor behaviors have led to the concept of layered processing in the spinal cord. It has been postulated that spinal V3 interneurons (INs) play multiple roles in locomotion, leading us to investigate whether they form layered microcircuits. Using patch-clamp recordings in combination with holographic glutamate uncaging, we demonstrate focal, layered modules, in which ventromedial V3 INs form synapses with one another and with ventrolateral V3 INs, which in turn form synapses with ipsilateral motoneurons. Motoneurons, in turn, provide recurrent excitatory, glutamatergic input to V3 INs. Thus, ventral V3 interneurons form layered microcircuits that could function to ensure well-timed, spatially specific movements. Two populations of ventral spinal V3 interneurons (INs) can be distinguished Medial (V3VMed) and lateral (V3VLat) populations differ in connectivity patterns Motoneuron axons recurrently excite ipsilateral V3 INs Ventral spinal V3 INs form layered microcircuits for motor output Using electrophysiology combined with holographic photostimulation, Chopek et al. demonstrate focal layered microcircuits within the spinal cord. These microcircuits are composed of two ventral V3 interneuron sub-populations and ipsilateral motoneurons. Synaptic connectivity was established from medial to lateral, with motoneurons recurrently exciting both V3 interneuron sub-populations.
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