Rest mutant zebrafish swim erratically and display atypical spatial preferences.
Rest mutant zebrafish swim erratically and display atypical spatial preferences.
复制标题
其余的突变斑马鱼游动不规律，并表现出非典型的空间偏好。
DOI：
10.1016/j.bbr.2015.02.026
复制
发表时间：
2015-05-01
影响因子：
2.7
通讯作者：
Sirotkin, Howard I.
中科院分区：
文献类型：
--
作者：
Moravec, Cara E.;Li, Edward;Maaswinkel, Hans;Kritzer, Mary F.;Weng, Wei;Sirotkin, Howard I.
Rest/Nrsf转录抑制因子调节大量神经特异性基因的表达。这些靶基因在神经系统发育、可塑性和突触发生等过程中发挥着重要作用。然而，rest介导的转录调控对行为的影响尚未得到充分研究，部分原因是小鼠基因敲除的胚胎致命性。为了研究斑马鱼在行为中对休息的需求，我们利用斑马鱼休息突变体来研究斑马鱼成虫和幼虫的一系列行为。成年休息突变体在新的环境中表现出异常行为，包括增加垂直游泳，不稳定的游泳模式和对水箱壁的倾向。成年雄性的繁殖成功率也有所下降。在受精后6天（dpf），休息的突变体幼虫活性降低，但对光和声刺激表现出正常的诱发反应。总的来说，这些结果提供了证据，证明休息功能障碍会导致成虫的非典型游泳模式和偏好，并减少幼虫的运动活动。本研究首次提供了rest突变体的行为分析，揭示了rest调节的特定行为。
The Rest/Nrsf transcriptional repressor modulates expression of a large set of neural specific genes. Many of these target genes have well characterized roles in nervous system processes including development, plasticity and synaptogenesis. However, the impact of Rest-mediated transcriptional regulation on behavior has been understudied due in part to the embryonic lethality of the mouse knockout. To investigate the requirement for Rest in behavior, we employed the zebrafish rest mutant to explore a range of behaviors in adults and larva. Adult rest mutants of both sexes showed abnormal behaviors in a novel environment including increased vertical swimming, erratic swimming patterns and a proclivity for the tank walls. Adult males also had diminished reproductive success. At 6 days post fertilization (dpf), rest mutant larva were hypoactive, but displayed normal evoked responses to light and sound stimuli. Overall, these results provide evidence that rest dysfunction produces atypical swimming patterns and preferences in adults, and reduced locomotor activity in larvae. This study provides the first behavioral analysis of rest mutants and reveals specific behaviors that are modulated by Rest.
登录
查看更多内容
影响因子：
2.7
作者：
Egan, Rupert J.;Bergner, Carisa L.;Hart, Peter C.;Cachat, Jonathan M.;Canavello, Peter R.;Elegante, Marco F.;Elkhayat, Salem I.;Bartels, Brett K.;Tien, Anna K.;Tien, David H.;Mohnot, Sopan;Beeson, Esther;Glasgow, Eric;Amri, Hakima;Zukowska, Zofia;Kalueff, Allan V.
通讯作者：
Kalueff, Allan V.
影响因子：
3.7
作者：
Cachat J;Stewart A;Utterback E;Hart P;Gaikwad S;Wong K;Kyzar E;Wu N;Kalueff AV
通讯作者：
Kalueff AV
DOI：
10.1016/j.bbagrm.2009.05.007
发表时间：
2009-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Grzenda A;Lomberk G;Zhang JS;Urrutia R
通讯作者：
Urrutia R
影响因子：
7
作者：
Mortazavi, Ali;Thompson, Evonne Chen Leeper;Wold, Barbara
通讯作者：
Wold, Barbara
影响因子：
64.5
作者：
CHONG, JHA;TAPIARAMIREZ, J;MANDEL, G
通讯作者：
MANDEL, G