Extracellular-vesicles delivered tumor-specific sequential nanocatalysts can be used for MRI-informed nanocatalytic Therapy of hepatocellular carcinoma.
Extracellular-vesicles delivered tumor-specific sequential nanocatalysts can be used for MRI-informed nanocatalytic Therapy of hepatocellular carcinoma.
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细胞外囊泡递送的肿瘤特异性连续纳米催化剂可用于肝细胞癌的 MRI 信息纳米催化治疗
作者：
Wu H;Xing H;Wu MC;Shen F;Chen Y;Yang T
背景：晚期肝细胞癌（HCC）的传统治疗策略仍然是一个巨大的挑战，因此迫切需要特异性和有效抑制 HCC 的替代治疗方式。方法：在这项工作中，HCC 衍生的细胞外囊泡 (EV) 被用作连续纳米催化剂 GOD-ESIONs@EVs (GE@EVs) 的表面纳米载体，用于针对 HCC 的肿瘤特异性和级联纳米催化治疗。通过精氨酸-甘氨酸-天冬氨酸（RGD）靶向效应和膜融合增强细胞内吞作用，连续纳米催化剂可以在更短的时间内对 HCC 肿瘤区域进行更有效的治疗。结果：负载的葡萄糖氧化酶（GOD）催化消耗葡萄糖过量产生过氧化氢（H2O2），ESIONs催化类芬顿反应产生剧毒羟自由基，在弱酸性肿瘤微环境下实现，从而刺激肝癌细胞凋亡和坏死。该策略证明了GE@EVs对HCC的高度主动靶向能力，在体外和体内实现了高效的肿瘤抑制。此外，合成的纳米反应器可以作为磁共振成像所需的纳米级造影剂，在连续的纳米催化处理过程中表现出理想的成像能力。结论：表面工程电动汽车的这种应用不仅证明了GE@EVs对HCC的高性能催化治疗方式，而且拓宽了电动汽车的多功能生物应用。
Background: Conventional therapeutic strategies for advanced hepatocellular carcinoma (HCC) remains a great challenge, therefore the alternative therapeutic modality for specific and efficient HCC suppression is urgently needed. Methods: In this work, HCC-derived extracellular vesicles (EVs) were applied as surface nanocarrier for sequential nanocatalysts GOD-ESIONs@EVs (GE@EVs) of tumor-specific and cascade nanocatalytic therapy against HCC. By enhancing the intracellular endocytosis through arginine-glycine-aspartic acid (RGD)-targeting effect and membrane fusion, sequential nanocatalysts led to more efficient treatment in the HCC tumor region in a shorter period of time. Results: Through glucose consumption as catalyzed by the loaded glucose oxidase (GOD) to overproduce hydrogen peroxide (H2O2), highly toxic hydroxyl radicals were generated by Fenton-like reaction as catalyzed by ESIONs, which was achieved under the mildly acidic tumor microenvironment, enabling the stimuli of the apoptosis and necrosis of HCC cells. This strategy demonstrated the high active-targeting capability of GE@EVs into HCC, achieving highly efficient tumor suppression both in vitro and in vivo. In addition, the as-synthesized nanoreactor could act as a desirable nanoscale contrast agent for magnetic resonance imaging, which exhibited desirable imaging capability during the sequential nanocatalytic treatment. Conclusion: This application of surface-engineering EVs not only proves the high-performance catalytic therapeutic modality of GE@EVs for HCC, but also broadens the versatile bio-applications of EVs.
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二维 MXene 的表面纳米孔工程用于肝细胞癌的靶向协同多疗法
影响因子：
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通过纳米催化剂递送，肿瘤选择性催化纳米医学。
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