Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways.
Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways.
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DOI：
10.1093/nar/gku273
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发表时间：
2014-06
影响因子：
14.9
通讯作者：
Grohmann D
中科院分区：
文献类型：
--
作者：
Gietl A;Holzmeister P;Blombach F;Schulz S;von Voithenberg LV;Lamb DC;Werner F;Tinnefeld P;Grohmann D
在转录起始过程中，启动子DNA被基础转录因子TATA结合蛋白(TBP)识别和弯曲。转录因子B(TFB)随后与TBP-DNA复合体结合，紧随其后的是核糖核酸聚合酶的招募，导致预起始复合体的形成。TBP和TFB/Tf(II)B在真核-古生物结构和功能上高度保守，但有趣的是它们必须在非常不同的条件下工作。利用单对荧光共振能量转移技术，我们实时监测了真核生物和古生菌在没有TFB和存在TFB时的DNA弯曲。我们观察到TBP-DNA相互作用的寿命在古生物和真核生物系统中有很大的不同。我们发现，真核DNA-TBP相互作用具有线性、阶梯式的弯曲机制，中间状态由不同的弯曲角度来区分。Tf(II)B特异性地稳定完全弯曲的TBP-启动子DNA复合体，我们将这一步骤确定为调节检查点。相比之下，古生菌的TBP-DNA相互作用是极其动态的，来自古生菌生物体Sulfolobus acidocaldarius的TBP严格要求TFB进行DNA弯曲。因此，我们证明了转录起始在形成预起始复合体的过程中遵循不同的途径。
During transcription initiation, the promoter DNA is recognized and bent by the basal transcription factor TATA-binding protein (TBP). Subsequent association of transcription factor B (TFB) with the TBP–DNA complex is followed by the recruitment of the ribonucleic acid polymerase resulting in the formation of the pre-initiation complex. TBP and TFB/TF(II)B are highly conserved in structure and function among the eukaryotic-archaeal domain but intriguingly have to operate under vastly different conditions. Employing single-pair fluorescence resonance energy transfer, we monitored DNA bending by eukaryotic and archaeal TBPs in the absence and presence of TFB in real-time. We observed that the lifetime of the TBP–DNA interaction differs significantly between the archaeal and eukaryotic system. We show that the eukaryotic DNA-TBP interaction is characterized by a linear, stepwise bending mechanism with an intermediate state distinguished by a distinct bending angle. TF(II)B specifically stabilizes the fully bent TBP–promoter DNA complex and we identify this step as a regulatory checkpoint. In contrast, the archaeal TBP–DNA interaction is extremely dynamic and TBP from the archaeal organism Sulfolobus acidocaldarius strictly requires TFB for DNA bending. Thus, we demonstrate that transcription initiation follows diverse pathways on the way to the formation of the pre-initiation complex.
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影响因子：
7.7
作者：
Bell, SD;Brinkman, AB;Jackson, SP
通讯作者：
Jackson, SP
影响因子：
10.5
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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DOI：
10.1098/rstb.2006.1841
发表时间：
2006-06-29
影响因子：
6.3
作者：
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通讯作者：
Brochier-Armanet, Celine