Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
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DOI：
10.1083/jcb.201304076
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发表时间：
2014-01-20
期刊：
影响因子：
--
通讯作者：
Hamada H
中科院分区：
文献类型：
--
作者：
Dong F;Shinohara K;Botilde Y;Nabeshima R;Asai Y;Fukumoto A;Hasegawa T;Matsuo M;Takeda H;Shiratori H;Nakamura T;Hamada H
Pih 1d 3促进轴丝外和内动力蛋白臂的稳定性和组装，以允许生精细胞中动力蛋白复合物的细胞质预组装。轴丝动力蛋白复合物在转运到纤毛之前在细胞质中预先组装，但这一过程的机制尚不清楚。我们现在发现，缺乏PIH 1结构域蛋白Pih 1d 3的小鼠发育正常，但表现出雄性不育。Pih 1d 3 −/−精子不动且脆弱，鞭毛的轴丝缺乏外动力蛋白臂（ODA）和内动力蛋白臂（IDA），显示出9+2微管结构紊乱。Pih 1d 3在生精细胞中特异性表达，其mRNA在粗线期精母细胞中最丰富。Pih 1d 3定位于生精细胞的细胞质中，但在精子细胞和成熟精子中未检测到。ODA和IDA蛋白质的水平在突变体睾丸和精子中降低，并且发现Pih 1d 3与ODA的中间链以及与Hsp 70和Hsp 90相互作用。我们的研究结果表明，Pih 1d 3有助于细胞质预组装的动力蛋白复合物在生精细胞中的稳定和促进复合物的形成，由ODA和IDA蛋白。
Pih1d3 promotes the stability and assembly of axonemal outer and inner dynein arms to allow for cytoplasmic preassembly of dynein complexes in spermatogenic cells. Axonemal dynein complexes are preassembled in the cytoplasm before their transport to cilia, but the mechanism of this process remains unclear. We now show that mice lacking Pih1d3, a PIH1 domain–containing protein, develop normally but manifest male sterility. Pih1d3−/− sperm were immotile and fragile, with the axoneme of the flagellum lacking outer dynein arms (ODAs) and inner dynein arms (IDAs) and showing a disturbed 9+2 microtubule organization. Pih1d3 was expressed specifically in spermatogenic cells, with the mRNA being most abundant in pachytene spermatocytes. Pih1d3 localized to the cytoplasm of spermatogenic cells but was not detected in spermatids or mature sperm. The levels of ODA and IDA proteins were reduced in the mutant testis and sperm, and Pih1d3 was found to interact with an intermediate chain of ODA as well as with Hsp70 and Hsp90. Our results suggest that Pih1d3 contributes to cytoplasmic preassembly of dynein complexes in spermatogenic cells by stabilizing and promoting complex formation by ODA and IDA proteins.
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影响因子：
7.8
作者：
Qin, Hongmin;Diener, Dennis R;Geimer, Stefan;Cole, Douglas G;Rosenbaum, Joel L
通讯作者：
Rosenbaum, Joel L
影响因子：
30.8
作者：
Mitchison, Hannah M.;Schmidts, Miriam;Loges, Niki T.;Freshour, Judy;Dritsoula, Athina;Hirst, Rob A.;O'Callaghan, Christopher;Blau, Hannah;Al Dabbagh, Maha;Olbrich, Heike;Beales, Philip L.;Yagi, Toshiki;Mussaffi, Huda;Chung, Eddie M. K.;Omran, Heymut;Mitchell, David R.
通讯作者：
Mitchell, David R.
影响因子：
64.8
作者：
Omran, Heymut;Kobayashi, Daisuke;Olbrich, Heike;Tsukahara, Tatsuya;Loges, Niki T.;Hagiwara, Haruo;Zhang, Qi;Leblond, Gerard;O'Toole, Eileen;Hara, Chikako;Mizuno, Hideaki;Kawano, Hiroyuki;Fliegauf, Manfred;Yagi, Toshiki;Koshida, Sumito;Miyawaki, Atsushi;Zentgraf, Hanswalter;Seithe, Horst;Reinhardt, Richard;Watanabe, Yoshinori;Kamiya, Ritsu;Mitchell, David R.;Takeda, Hiroyuki
通讯作者：
Takeda, Hiroyuki
DOI：
10.1083/jcb.41.2.600
发表时间：
1969-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Rosenbaum JL;Moulder JE;Ringo DL
通讯作者：
Ringo DL
影响因子：
4.8
作者：
Takaki, Eiichi;Fujimoto, Mitsuaki;Nakai, Akira
通讯作者：
Nakai, Akira