Effects of the Thyroid Endocrine System on Gonadal Sex Ratios and Sex-Related Gene Expression in the Pufferfish Takifugu rubripes.
Effects of the Thyroid Endocrine System on Gonadal Sex Ratios and Sex-Related Gene Expression in the Pufferfish Takifugu rubripes.
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甲状腺内分泌系统对红鳍东方鲀性腺性别比及性别相关基因表达的影响
DOI：
10.3389/fendo.2021.674954
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发表时间：
2021
影响因子：
5.2
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Yuan Z;Shen X;Yan H;Jiang J;Liu B;Zhang L;Wu Y;Liu Y;Liu Q
为了探讨甲状腺激素对性腺性别分化的影响和机制，在孵化后25至80天（dah）内，用甲状腺素（甲巯咪唑，MET，1000 g/g）和甲状腺素（T4，2 nM）处理红鳍东方鲀幼虫。性腺组织学和性别比例的鱼，然后确定在80日龄。MET处理诱导T细胞雄性化，而T4处理不诱导T细胞雌性化。红足幼虫然后在80天时进行性腺的转录组学分析。在组间大量差异表达的基因中，foxl 2、cyp 19 a1 a和dmrt 1的表达发生了改变。在处理后25、40、55天（dat）进一步通过qPCR分析foxl 2、cyp 19 a1 a、dmrt 1和gsdf的表达。MET处理抑制了foxl 2和cyp 19 a1 a的表达，诱导了dmrt 1的表达（p < 0.05）。此外，T4治疗诱导的cyp 19 a1 a基因XY性腺的表达增加，只有在25大。然而，cyp 19 a1 a表达的增加没有继续到40和55大。这可能解释了为什么雌性化的幼虫没有发现在T4处理组。因此，本研究提供了第一个证据，MET治疗导致硬骨鱼雄性化。MET诱导的T.红足可能主要通过抑制FOXL 2和CYP 19 A 1A的表达以及刺激DMRT 1的表达而起作用。此外，高浓度T_4或不同浓度T_3对性别分化的影响还有待进一步研究。
To examine the effect and mechanism of thyroid hormone on gonadal sex differentiation, Takifugu rubripes larvae were treated with goitrogen (methimazole, MET, 1000 g/g), and thyroxine (T4, 2nM) from 25 to 80 days after hatching (dah). Gonadal histology and sex ratios of fish were then determined at 80 dah. MET treatment induced masculinization, but T4 treatment did not induce feminization in T. rubripes larvae. Transcriptomic analysis of gonads at 80 dah was then conducted. Among the large number of differentially expressed genes between the groups, the expression of foxl2, cyp19a1a, and dmrt1 was altered. The expression of foxl2, cyp19a1a, dmrt1 and gsdf at 25, 40, 55 days after treatment (dat) was further analyzed by qPCR. MET treatment suppressed the expression of foxl2 and cyp19a1a, and induced the expression of dmrt1 in genetic females (p < 0.05). Additionally, T4 treatment induced an increase in the expression of cyp19a1a in genetic XY gonads only at 25 dat. However, the increase in cyp19a1a expression did not continue to 40 and 55 dat. This may explain why feminization of larvae was not found in the T4-treated group. Thus, the present study provides the first evidence that MET treatment causes masculinization in teleost fish. The effects of MET-induced masculinization in T. rubripes may act primarily via suppression of the expression of foxl2 and cyp19a1a, and stimulation of the expression of dmrt1. Moreover, the effects of higher concentrations of T4 or different concentrations of T3, on sex differentiation require further testing.
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DOI：
10.1016/j.bbrc.2015.09.112
发表时间：
2015-11-06
影响因子：
3.1
作者：
Imai, Takuto;Saino, Kentaro;Matsuda, Masaru
通讯作者：
Matsuda, Masaru
影响因子：
2.7
作者：
Campbell, Diana E. K.;Langlois, Valerie S.
通讯作者：
Langlois, Valerie S.
影响因子：
3.7
作者：
Hattori RS;Fernandino JI;Kishii A;Kimura H;Kinno T;Oura M;Somoza GM;Yokota M;Strüssmann CA;Watanabe S
通讯作者：
Watanabe S
影响因子：
4.5
作者：
Fenske, M;Segner, H
通讯作者：
Segner, H
影响因子：
4.1
作者：
Bernhardt, Richard R.;von Hippel, Frank A.;Cresko, William A.
通讯作者：
Cresko, William A.