Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations.
Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations.
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细胞动力学的配位有助于牙齿上皮变形。
DOI：
10.1371/journal.pone.0161336
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Tsuji T
中科院分区：
文献类型：
--
作者：
Morita R;Kihira M;Nakatsu Y;Nomoto Y;Ogawa M;Ohashi K;Mizuno K;Tachikawa T;Ishimoto Y;Morishita Y;Tsuji T
外胚层器官的形态是由几种变形模式的适当组合形成的，例如内陷和各向异性组织伸长。然而，多细胞动力学在变形过程中是如何协调的仍有待阐明。在这里，我们开发了一个四维(4D)分析系统，用于跟踪发育中的牙齿上皮细胞在单细胞分辨率下的运动和分裂。Fucci探针的表达模式阐明了牙胚中特定区域和阶段的细胞周期模式，这与通过组织水平变形分析估计的体积增长率模式非常一致。在生长速度较快的区域，细胞的运动性较高，而有丝分裂方向明显偏向于上皮中组织伸长的方向。此外，细胞动力学和组织水平变形的这些时空模式与肌动蛋白解聚因子cofilin的活性高度相关，表明通过肌动蛋白重塑来协调细胞动力学在牙齿上皮形态发生中起重要作用。我们的系统增强了对细胞行为在外胚层器官发生过程中如何协调的理解，这在组织学分析中是观察不到的。
The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dimensional (4D) analysis system for tracking cell movement and division at a single-cell resolution in developing tooth epithelium. The expression patterns of a Fucci probe clarified the region- and stage-specific cell cycle patterns within the tooth germ, which were in good agreement with the pattern of the volume growth rate estimated from tissue-level deformation analysis. Cellular motility was higher in the regions with higher growth rates, while the mitotic orientation was significantly biased along the direction of tissue elongation in the epithelium. Further, these spatio-temporal patterns of cellular dynamics and tissue-level deformation were highly correlated with that of the activity of cofilin, which is an actin depolymerization factor, suggesting that the coordination of cellular dynamics via actin remodeling plays an important role in tooth epithelial morphogenesis. Our system enhances the understanding of how cellular behaviors are coordinated during ectodermal organogenesis, which cannot be observed from histological analyses.
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果蝇胃肠道的动态分析提供了对集体细胞迁移的见解。
DOI：
10.1126/science.1167094
发表时间：
2008-12-05
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
48
作者：
Nakao, Kazuhisa;Morita, Ritsuko;Tsuji, Takashi
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DOI：
10.1083/jcb.136.6.1307
发表时间：
1997-03-24
期刊：
The Journal of cell biology
影响因子：
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作者：
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通讯作者：
Pantaloni D
DOI：
10.1073/pnas.0902944106
发表时间：
2009-08-11
影响因子：
11.1
作者：
Ikeda, Etsuko;Morita, Ritsuko;Tsuji, Takashi
通讯作者：
Tsuji, Takashi