K-Carrageenan Stimulates Pre-Osteoblast Proliferation and Osteogenic Differentiation: A Potential Factor for the Promotion of Bone Regeneration?
K-Carrageenan Stimulates Pre-Osteoblast Proliferation and Osteogenic Differentiation: A Potential Factor for the Promotion of Bone Regeneration?
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DOI：
10.3390/molecules26206131
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发表时间：
2021-10-11
期刊：
影响因子：
--
通讯作者：
Klein-Nulend J
中科院分区：
文献类型：
--
作者：
Cao W;Jin J;Wu G;Bravenboer N;Helder MN;Pathak JL;Zandieh-Doulabi B;Hogervorst JMA;Matsukawa S;Geonzon LC;Bacabac RG;Schulten EAJM;Klein-Nulend J
目前基于细胞的骨组织再生策略不能覆盖大的骨缺损。角叉菜胶是一种具有高度亲水性和生物相容性的海藻硫酸多糖，已被提出作为一种有前途的候选组织工程应用。κ-角叉菜胶是否可用于增强骨再生仍不清楚。本研究旨在通过检测κ-卡拉胶对成骨细胞前体增殖和成骨分化的影响，探讨κ-卡拉胶是否具有成骨潜能。用κ-角叉菜胶（0.5和2 mg/mL）处理在1小时增加MC 3 T3-E1前成骨细胞粘附和铺展。K-角叉菜胶（0.125-2 mg/mL）剂量依赖性地增加前成骨细胞增殖和代谢活性，在第三天在2 mg/mL时具有最大效果。K-角叉菜胶（0.5和2 mg/mL）增加成骨分化，如第4天碱性磷酸酶活性增强（2 mg/mL时增加1.8倍）和第21天基质矿化（2 mg/mL时增加6.2倍）所示。在第14天和第21天，K-角叉菜胶增强成骨基因表达（Opn、Dmp 1和Mepe）。综上所述，κ-卡拉胶可促进MC 3 T3-E1前成骨细胞的粘附和铺展、代谢活性、增殖和成骨分化，提示κ-卡拉胶是一种潜在的成骨诱导因子，可用于临床促进骨再生。
Current cell-based bone tissue regeneration strategies cannot cover large bone defects. K-carrageenan is a highly hydrophilic and biocompatible seaweed-derived sulfated polysaccharide, that has been proposed as a promising candidate for tissue engineering applications. Whether κ-carrageenan can be used to enhance bone regeneration is still unclear. In this study, we aimed to investigate whether κ-carrageenan has osteogenic potential by testing its effect on pre-osteoblast proliferation and osteogenic differentiation in vitro. Treatment with κ-carrageenan (0.5 and 2 mg/mL) increased both MC3T3-E1 pre-osteoblast adhesion and spreading at 1 h. K-carrageenan (0.125–2 mg/mL) dose-dependently increased pre-osteoblast proliferation and metabolic activity, with a maximum effect at 2 mg/mL at day three. K-carrageenan (0.5 and 2 mg/mL) increased osteogenic differentiation, as shown by enhanced alkaline phosphatase activity (1.8-fold increase at 2 mg/mL) at day four, and matrix mineralization (6.2-fold increase at 2 mg/mL) at day 21. K-carrageenan enhanced osteogenic gene expression (Opn, Dmp1, and Mepe) at day 14 and 21. In conclusion, κ-carrageenan promoted MC3T3-E1 pre-osteoblast adhesion and spreading, metabolic activity, proliferation, and osteogenic differentiation, suggesting that κ-carrageenan is a potential osteogenic inductive factor for clinical application to enhance bone regeneration.
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作者：
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影响因子：
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