Auxin production in diploid microsporocytes is necessary and sufficient for early stages of pollen development.
Auxin production in diploid microsporocytes is necessary and sufficient for early stages of pollen development.
复制标题
二倍体小孢子母细胞中生长素的产生对于花粉发育的早期阶段是必要且充分的
DOI：
10.1371/journal.pgen.1007397
复制
发表时间：
2018-05
期刊：
影响因子：
4.5
通讯作者：
Yang ZN
中科院分区：
文献类型：
--
作者：
Yao X;Tian L;Yang J;Zhao YN;Zhu YX;Dai X;Zhao Y;Yang ZN
拟南芥配子体发育取决于孢子体细胞的营养物质和细胞壁材料。然而，尚不清楚配子体发育是否也需要来自孢子体组织的激素和信号分子。在此，我们表明，孢子体小孢子母细胞中黄素单加氧酶 YUC2 和 YUC6 产生的生长素对于花粉发育的早期阶段至关重要。单倍体小孢子的第一次不对称有丝分裂（PMI）是雄配子体发育中最早的事件。 yuc2yuc6 双突变体中的小孢子发育在 PMI 之前停止，因此 yuc2yuc6 无法产生有活力的花粉。我们的遗传分析表明，YUC2 和 YUC6 作为花粉形成的孢子体基因。我们进一步表明，绒毡层中生长素的异位产生为花粉发育提供营养，但未能挽救 yuc2yuc6 的不育表型。相比之下，小孢子母细胞或小孢子中生长素的产生挽救了 yuc2yuc6 双突变体的花粉发育缺陷。我们的结果表明，孢子体小孢子细胞和小孢子中的局部生长素生物合成在从孢子体到雄配子体的世代过渡过程中控制雄配子体的发育。
Gametophytic development in Arabidopsis depends on nutrients and cell wall materials from sporophytic cells. However, it is not clear whether hormones and signaling molecules from sporophytic tissues are also required for gametophytic development. Herein, we show that auxin produced by the flavin monooxygenases YUC2 and YUC6 in the sporophytic microsporocytes is essential for early stages of pollen development. The first asymmetric mitotic division (PMI) of haploid microspores is the earliest event in male gametophyte development. Microspore development in yuc2yuc6 double mutants arrests before PMI and consequently yuc2yuc6 fail to produce viable pollens. Our genetic analyses reveal that YUC2 and YUC6 act as sporophytic genes for pollen formation. We further show that ectopic production of auxin in tapetum, which provides nutrients for pollen development, fails to rescue the sterile phenotypes of yuc2yuc6. In contrast, production of auxin in either microsporocytes or microspores rescued the defects of pollen development in yuc2yuc6 double mutants. Our results demonstrate that local auxin biosynthesis in sporophytic microsporocytic cells and microspore controls male gametophyte development during the generation transition from sporophyte to male gametophyte.
登录
查看更多内容
影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
Sumner, Lloyd W.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde
影响因子：
11.4
作者：
Cecchetti, Valentina;Brunetti, Patrizia;Cardarelli, Maura
通讯作者：
Cardarelli, Maura
影响因子：
12.3
作者：
Honys D;Twell D
通讯作者：
Twell D