Vibrational Spectroscopic Detection of a Single Virus by Mid-Infrared Photothermal Microscopy.
Vibrational Spectroscopic Detection of a Single Virus by Mid-Infrared Photothermal Microscopy.
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DOI：
10.1021/acs.analchem.0c05333
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发表时间：
2021-03-02
影响因子：
7.4
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Zhang, Yi;Yurdakul, Celalettin;Devaux, Alexander J.;Wang, Le;Xu, Xiaoji G.;Connor, John H.;Unlu, M. Selim;Cheng, Ji-Xin
我们报告了一个共焦干涉中红外光热（MIP）显微镜的超灵敏和空间分辨的单个病毒的化学成像。利用干涉散射原理，对化学键红外吸收引起的微弱光热信号进行了探测。光谱MIP检测单个水泡性口炎病毒（VSV）和痘病毒的证明。单病毒谱在同一病毒类型内显示出高度一致性。主要光谱峰由归因于病毒蛋白的酰胺I和酰胺II振动贡献。这两个峰的比率在VSV和痘病毒之间是显著不同的，突出了使用干涉MIP显微镜用于病毒颗粒的无标记区分的潜力。这种全光学化学成像方法为以无标记方式进行生物纳米颗粒的光谱检测开辟了新的途径，并可能有助于预测和控制新出现的病毒株的爆发。
We report a confocal interferometric mid-infrared photothermal (MIP) microscope for ultra-sensitive and spatially resolved chemical imaging of individual viruses. The interferometric scattering principle is applied to detect the very weak photothermal signal induced by infrared absorption of chemical bonds. Spectroscopic MIP detection of single vesicular stomatitis viruses (VSVs) and poxviruses is demonstrated. The single virus spectra show high consistency within the same virus type. The dominant spectral peaks are contributed by the amide I and amide II vibrations attributed to the viral proteins. The ratio of these two peaks is significantly different between VSVs and poxviruses, highlighting the potential of using interferometric MIP microscopy for label-free differentiation of viral particles. This all-optical chemical imaging method opens a new way for spectroscopic detection of biological nanoparticles in a label-free manner and may facilitate in predicting and controlling outbreaks of emerging virus strains.
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影响因子：
48
作者：
Hu, Fanghao;Shi, Lixue;Min, Wei
通讯作者：
Min, Wei
通过拉曼集成中红外光热显微镜对活细胞进行指纹识别
影响因子：
7.4
作者：
Li, Xiaojie;Zhang, Delong;Cheng, Ji-Xin
通讯作者：
Cheng, Ji-Xin
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Baize, Sylvain;Pannetier, Delphine;Guenther, Stephan
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Guenther, Stephan
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作者：
Bai, Yeran;Zhang, Delong;Cheng, Ji-Xin
通讯作者：
Cheng, Ji-Xin
影响因子：
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作者：
Gaiduk, A.;Yorulmaz, M.;Orrit, M.
通讯作者：
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