Genome-wide identification of multifunctional laccase gene family in cotton (Gossypium spp.); expression and biochemical analysis during fiber development.
Genome-wide identification of multifunctional laccase gene family in cotton (Gossypium spp.); expression and biochemical analysis during fiber development.
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棉中多功能漆酶基因家族的全基因组鉴定（Gossypium spp。）；纤维发育过程中的表达和生化分析。
DOI：
10.1038/srep34309
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发表时间：
2016-09-29
影响因子：
4.6
通讯作者：
Mendu V
中科院分区：
文献类型：
--
作者：
Balasubramanian VK;Rai KM;Thu SW;Hii MM;Mendu V
由种皮表皮细胞产生的单细胞棉纤维是纺织纤维的最大天然来源。棉纤维的经济价值在于它的长度和质量。多功能漆酶在植物细胞伸长、木质化和色素沉淀等方面起着重要作用，对棉纤维品质也有重要影响。对栽培的异源四倍体（G. hirsutum）及其祖先二倍体（G. arboreum和G. raimondii）棉花进行全基因组分析，分别鉴定出84个、44个和46个漆酶基因。染色体定位、系统发育、保守结构域和物理性质分析表明，漆酶在3种棉花中具有高度的保守性。对发育中的棉纤维进行了基因表达、酶活性和生化分析。44个漆酶中，40个漆酶在纤维发育的不同阶段均有表达。25 DPA时，漆酶活性越高，木质素含量越高。此外，对棉纤维酚类化合物的分析表明，在25 DPA时，这些化合物整体减少，表明这些底物可能在次生细胞壁生物合成过程中掺入木质素聚合物。总体数据表明，漆酶在棉纤维发育中起着重要作用，为控制纤维发育和质量提供了极好的机会。
The single-celled cotton fibers, produced from seed coat epidermal cells are the largest natural source of textile fibers. The economic value of cotton fiber lies in its length and quality. The multifunctional laccase enzymes play important roles in cell elongation, lignification and pigmentation in plants and could play crucial role in cotton fiber quality. Genome-wide analysis of cultivated allotetraploid (G. hirsutum) and its progenitor diploid (G. arboreum and G. raimondii) cotton species identified 84, 44 and 46 laccase genes, respectively. Analysis of chromosomal location, phylogeny, conserved domain and physical properties showed highly conserved nature of laccases across three cotton species. Gene expression, enzymatic activity and biochemical analysis of developing cotton fibers was performed using G. arboreum species. Of the total 44, 40 laccases showed expression during different stages of fiber development. The higher enzymatic activity of laccases correlated with higher lignin content at 25 DPA (Days Post Anthesis). Further, analysis of cotton fiber phenolic compounds showed an overall decrease at 25 DPA indicating possible incorporation of these substrates into lignin polymer during secondary cell wall biosynthesis. Overall data indicate significant roles of laccases in cotton fiber development, and presents an excellent opportunity for manipulation of fiber development and quality.
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影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
3.7
作者：
Feng H;Tian X;Liu Y;Li Y;Zhang X;Jones BJ;Sun Y;Sun J
通讯作者：
Sun J
影响因子：
4.9
作者：
Hernandez-Gomez MC;Runavot JL;Guo X;Bourot S;Benians TA;Willats WG;Meulewaeter F;Knox JP
通讯作者：
Knox JP
影响因子：
3.8
作者：
Dittmer, NT;Suderman, RJ;Kanost, MR
通讯作者：
Kanost, MR
影响因子：
11.6
作者：
Brown, DM;Zeef, LAH;Turner, SR
通讯作者：
Turner, SR