Detection of phosphatidylethanol (PEth) in the blood of drivers in an alcohol ignition interlock program.
Detection of phosphatidylethanol (PEth) in the blood of drivers in an alcohol ignition interlock program.
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DOI：
10.1080/15389588.2010.544048
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发表时间：
2011-04
影响因子：
2
通讯作者：
Jones M
中科院分区：
文献类型：
--
作者：
Marques P;Hansson T;Isaksson A;Walther L;Jones J;Lewis D;Jones M
连锁血液酒精浓度(BAC)测试的失败率是连锁后再次犯罪的强烈预测因素，也是酒精使用的部分替代指标。众所周知，在联锁计划开始时测量的酒精生物标志物与联锁使用几个月的BAC测试失败率有很好的相关性。这项研究评估了两种测量低血生物标记物pEH(磷脂酰乙醇)水平的方法。PEH是一种100%酒精特异性生物标记物，与酒后驾车风险的几个独立指标密切相关，包括8个其他生物标记物，3个心理测量评估，以及在连续使用多个月期间联锁BAC测试的失败率。有没有一种更灵敏的方法在程序进入时检测到饮酒，即使是那些后来没有记录失败的联锁测试的人？在281份驾驶员血液样本中，用高效液相色谱(HPLC)和液相色谱串联质谱仪(LCMMS)测定了PEH，以比较其敏感性和准确性。联锁BAC试验的平均失败率是衡量标志物敏感性的标准。LCMSM被校准为检测低水平饮酒作为违反禁欲的可能措施，并与标准的高效液相色谱(HPLC法)进行比较，结果显示两种方法显示出良好的定量关系(R2>；.86)。LCMMS在样品中检测到阳性的peth水平，低于高效液相色谱方法的检测限值。在酒后驾车违法者中，LCMSMS测量的PEH阳性比例高于高效液相色谱检测，这些违法者没有记录任何联锁BAC测试。尽管高效液相色谱法是临床酒精中毒样本中广泛使用的检测peth的标准，但当LCMSMS方法经过校准以检测微量的peth主要成分时，可以检测到接近零摄入量的酒精戒断程度，并且仍然与其他与酒精使用和道路安全相关的指标密切相关。
The rate of failed interlock blood alcohol concentration (BAC) tests is a strong predictor of recidivism post-interlock and a partial proxy for alcohol use. Alcohol biomarkers measured at the start of an interlock program are known to correlate well with rates of failed BAC tests over months of interlock use. This study evaluates two methods of measuring low blood levels of the biomarker PEth (phosphatidylethanol). PEth is a 100% alcohol specific biomarker and strongly intercorrelated with several independent indicators of drinking driving risk, including 8 other biomarkers, 3 psychometric assessments, and the rate of failed interlock BAC tests during many months of interlock use. Does a more sensitive method of measuring PEth at program entry detect drinking even among those who subsequently log no failed interlock tests? In a sample of 281 driver blood samples, PEth was measured by both high-performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LCMSMS) in order to compare sensitivity and accuracy. The average rate of failed interlock BAC tests was the criterion measure for marker sensitivity. LCMSMS, calibrated to detect low levels of drinking as a possible measure of abstinence violation, was judged relative to the standard HPLC assay for PEth measured up to 4 µmol/L. The two methods showed a good quantitative relationship (r2>.86). LCMSMS detected positive PEth levels in samples that were below the limit of detection of the HPLC method. PEth measured by LCMSMS was positive for a higher proportion of DUI offenders who logged zero failed interlock BAC tests than were detected by HPLC. Although HPLC is the widely used standard for measuring PEth in clinical alcoholism samples, the LCMSMS method, when calibrated to detect trace amounts of the major component of PEth, can detect abstinence levels of alcohol near zero intake and still correlate strongly with other indicators related to alcohol use and road safety.
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DOI：
10.1111/j.1530-0277.1986.tb05073.x
发表时间：
1986-04-01
期刊：
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
GJERDE, H;SAKSHAUG, J;MORLAND, J
通讯作者：
MORLAND, J
影响因子：
4.2
作者：
Appenzeller, BMR;Schneider, S;Wennig, R
通讯作者：
Wennig, R
影响因子：
4.2
作者：
Portman, M.;Penttila, A.;Kuoppasalmi, K.
通讯作者：
Kuoppasalmi, K.
DOI：
10.15288/jsa.2003.64.83
发表时间：
2003-01-01
期刊：
JOURNAL OF STUDIES ON ALCOHOL
影响因子：
--
作者：
Marques, PR;Tippetts, AS;Voas, RB
通讯作者：
Voas, RB
影响因子：
6
作者：
Bjerre, Bo;Marques, Paul;Thorsson, Ulf
通讯作者：
Thorsson, Ulf