Biodistribution, pharmacokinetics, and blood compatibility of native and PEGylated tobacco mosaic virus nano-rods and -spheres in mice.
Biodistribution, pharmacokinetics, and blood compatibility of native and PEGylated tobacco mosaic virus nano-rods and -spheres in mice.
复制标题
DOI：
10.1016/j.virol.2013.10.035
复制
发表时间：
2014-01-20
期刊：
影响因子：
3.7
通讯作者：
Steinmetz NF
中科院分区：
文献类型：
--
作者：
Bruckman MA;Randolph LN;VanMeter A;Hern S;Shoffstall AJ;Taurog RE;Steinmetz NF
了解纳米粒的药代动力学、血液相容性、生物分布和清除特性对于将其转化为临床应用具有重要意义。本文报道了烟草花叶病毒（TMV）在300×18 nm棒状和54 nm球状病毒中的生物分布和药动学特性。由相同蛋白质制成的棒和球的可用性提供了独特的支架来研究纳米颗粒形状对体内命运的影响。为了增强生物相容性，我们还考虑了PEG化制剂。总体而言，纳米颗粒的版本表现出相当的体内分布;注意到一些差异：数据表明，杆循环时间长于球体，说明形状对循环的影响。此外，PEG化增加了循环时间。我们发现，在肝脏和脾脏中的巨噬细胞清除TMV杆和球从循环。在脾脏中，病毒纳米颗粒在最终共同定位于B细胞滤泡之前通过边缘区运输。在几天内从肝脏和脾脏中清除TMV杆状和球状物，组织学无明显变化，注意到球状物与杆状物相比更快地从组织中清除。此外，血液生物相容性得到支持，因为没有制剂诱导凝血或溶血。这项工作为TMV在生物医学应用中的进一步应用和定制奠定了基础。
Understanding the pharmacokinetics, blood compatibility, biodistribution and clearance properties of nanoparticles is of great importance to their translation to clinical application. In this paper we report the biodistribution and pharmacokinetic properties of tobacco mosaic virus (TMV) in the forms of 300×18 nm rods and 54 nm-sized spheres. The availability of rods and spheres made of the same protein provides a unique scaffold to study the effect of nanoparticle shape on in vivo fate. For enhanced biocompatibility, we also considered a PEGylated formulation. Overall, the versions of nanoparticles exhibited comparable in vivo profiles; a few differences were noted: data indicate that rods circulate longer than spheres, illustrating the effect that shape plays on circulation. Also, PEGylation increased circulation times. We found that macrophages in the liver and spleen cleared the TMV rods and spheres from circulation. In the spleen, the viral nanoparticles trafficked through the marginal zone before eventually co-localizing in B-cell follicles. TMV rods and spheres were cleared from the liver and spleen within days with no apparent changes in histology, it was noted that spheres are more rapidly cleared from tissues compared to rods. Further, blood biocompatibility was supported, as none of the formulations induced clotting or hemolysis. This work lays the foundation for further application and tailoring of TMV for biomedical applications.
登录
查看更多内容
影响因子：
5.5
作者：
FITCHEN, J;BEACHY, RN;HEIN, MB
通讯作者：
HEIN, MB
影响因子：
3.2
作者：
Bruckman, Michael A.;Kaur, Gagandeep;Wang, Qian
通讯作者：
Wang, Qian
金纳米颗粒的几何形状和表面特征会影响其生物分布和巨噬细胞的吸收。
DOI：
10.1016/j.ejpb.2010.11.010
发表时间：
2011-04
期刊：
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
影响因子：
--
作者：
Arnida;Janát-Amsbury MM;Ray A;Peterson CM;Ghandehari H
通讯作者：
Ghandehari H
影响因子：
3.8
作者：
Atabekov, Joseph;Nikitin, Nikolai;Karpova, Olga
通讯作者：
Karpova, Olga
Auroshell 颗粒（金-二氧化硅纳米壳）静脉给药的毒性评价
影响因子：
2.2
作者：
Gad, Shayne C.;Sharp, Kelly L.;Goodrich, Glenn P.
通讯作者：
Goodrich, Glenn P.