A guide to analysis of mouse energy metabolism.

A guide to analysis of mouse energy metabolism.
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DOI:
10.1038/nmeth.1806
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发表时间:
2011-12-28
期刊:
影响因子:
48
通讯作者:
Ravussin, Eric
Ravussin, Eric
中科院分区:
生物学1区
文献类型:
--
作者:
Tschoep, Matthias H.;Speakman, John R.;Arch, Jonathan R. S.;Auwerx, Johan;Bruening, Jens C.;Chan, Lawrence;Eckel, Robert H.;Farese, Robert V., Jr.;Galgani, Jose E.;Hambly, Catherine;Herman, Mark A.;Horvath, Tamas L.;Kahn, Barbara B.;Kozma, Sara C.;Maratos-Flier, Eleftheria;Mueller, Timo D.;Muenzberg, Heike;Pfluger, Paul T.;Plum, Leona;Reitman, Marc L.;Rahmouni, Kamal;Shulman, Gerald I.;Thomas, George;Kahn, C. Ronald;Ravussin, Eric

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我们对小鼠模型能量代谢测量设计研究的复杂性和挑战提出了综合看法,包括评估能量消耗、能量摄入和身体成分及其统计分析的实用指南。我们希望本指南能够促进研究之间的比较,并最大限度地减少对数据的虚假解释。我们建议按体重或去脂体重划分能量消耗数据,并以直方图的形式呈现群体效应,而应通过绘制个体数据并使用协方差分析 (ANCOVA) 分析群体和身体成分效应来代替。
We present a consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof. We hope this guide will facilitate comparisons across studies and minimize spurious interpretations of data. We recommend that division of energy expenditure data by either body weight or lean body weight and that presentation of group effects as histograms should be replaced by plotting individual data and analyzing both group and body-composition effects using analysis of covariance (ANCOVA).
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