A transferrinlike (hemiferrin) mRNA is expressed in the germ cells of rat testis
A transferrinlike (hemiferrin) mRNA is expressed in the germ cells of rat testis
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大鼠睾丸生殖细胞中表达转铁蛋白样（半铁蛋白）mRNA
DOI：
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发表时间：
1991
影响因子：
5.3
通讯作者：
'. Griswold
中科院分区：
文献类型：
--
作者：
B. Stallard;M. Collard;D. Michael;'. Griswold
在睾丸中，被血睾丸屏障从血清中分离出来的生殖细胞主要依靠支持细胞来获取营养。例如，支持细胞合成的转铁蛋白在将铁从血清输送到发育中的生殖细胞中起着重要作用。由于其在睾丸中的作用，支持细胞转铁蛋白和mRNA已被广泛研究。通过对大鼠睾丸组织的RNA印迹分析，我们检测到一个2.6 kb的转铁蛋白转录本。此外，我们还检测到一个0.9 kb的新mRNA，其序列与转铁蛋白的3'端相似。这种0.9 kb的mRNA存在于生殖细胞中，但不存在于支持细胞、肝脏或大脑中。这种mRNA在睾丸中的主要来源是圆形精子。cDNA克隆序列分析表明，该mRNA编码的蛋白序列与转铁蛋白羧基端相似。多体分析表明，该转录本被翻译，因此可能在生殖细胞铁代谢中具有重要意义。讨论了转铁蛋白样mRNA生殖细胞与导致转铁蛋白不同结合的基因复制事件之间的进化意义。
In the testis, germ cells which are separated from the serum by the blood-testis barrier rely primarily on the Sertoli cell to obtain nutrients. For example, transferrin synthesized by the Sertoli cell is important in delivering iron from the serum to the developing germ cells. Because of its role in the testis, Sertoli cell transferrin protein and mRNA have been extensively studied. By using RNA blot analysis of rat testicular tissue, we detected a transcript of 2.6 kb which is attributed to transferrin. In addition, we detected a novel mRNA of 0.9 kb which had sequence similarity to the 3' end of transferrin. This 0.9-kb mRNA was present in germ cells, but not Sertoli cells, liver, or brain. The primary source of this mRNA in the testis was round spermatids. Sequence analysis of a cDNA clone showed that this mRNA encoded a protein with sequence similarity to the carboxy terminus of transferrin. Polysome analysis indicated that this transcript was translated and may therefore have importance in the iron metabolism of germ cells. The evolutionary implications between the transferrinlike mRNA germ cells and the gene duplication event which resulted in the diferric binding of transferrin are discussed.
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DOI：
10.1073/pnas.86.20.7741
发表时间：
1989-10-01
影响因子：
11.1
作者：
EHLERS, MRW;FOX, EA;RIORDAN, JF
通讯作者：
RIORDAN, JF
DOI：
10.1016/s0021-9258(18)43419-9
发表时间：
1980-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Skinner;M. Griswold
通讯作者：
M. Skinner;M. Griswold
DOI：
--
发表时间：
1989-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ravi S. Kumar;J. Kusari;S. Roy;R. Soffer;Ganes C. Sen
通讯作者：
Ravi S. Kumar;J. Kusari;S. Roy;R. Soffer;Ganes C. Sen
DOI：
10.1089/dna.1983.2.329
发表时间：
1983-01-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
CATHALA, G;SAVOURET, JF;BAXTER, JD
通讯作者：
BAXTER, JD