Lignocellulolytic Enzyme Production from Wood Rot Fungi Collected in Chiapas, Mexico, and Their Growth on Lignocellulosic Material.
Lignocellulolytic Enzyme Production from Wood Rot Fungi Collected in Chiapas, Mexico, and Their Growth on Lignocellulosic Material.
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DOI：
10.3390/jof7060450
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发表时间：
2021-06-05
期刊：
影响因子：
--
通讯作者：
Enciso-Sáenz S
中科院分区：
文献类型：
--
作者：
Sánchez-Corzo LD;Álvarez-Gutiérrez PE;Meza-Gordillo R;Villalobos-Maldonado JJ;Enciso-Pinto S;Enciso-Sáenz S
木材腐烂真菌的特征在于木质素分解和水解酶，其通过非特异性氧化和水解反应起作用。本工作的目的是评估生产的木质纤维素分解酶从收集的真菌，并分析其生长的木质纤维素材料。该研究考虑了从墨西哥恰帕斯州收集的18个物种，通过分类和分子技术鉴定，发现了11个不同的科。在以非洲棕榈壳（PH）、咖啡壳（CH）、松木锯屑（PS）和葡萄糖作为对照的培养基中获得每种分离物的生长速率，用ImageJ软件中分析的图像测量每日生长，发现CH培养基中的最高生长速率。测定纤维素酶、木聚糖酶和锰过氧化物酶（MnP）活性的效力指数（PI），以及木质素过氧化物酶（LiP）的定量，用菌株Phlebiopsis flavidoalba TecNM-ITTG L20-19和脏腐革菌TecNM-ITTG L32-1- 19是水解酶活性PI最高的菌株，纤维素酶PI分别为2.01和1.83，木聚糖酶PI分别为1.95和2.24，而黄白脉活菌TecNM-ITTG L20-19和血红栓菌TecNM-ITTG L14-19在LiP活性为7115 U/L时具有最高的氧化酶活性，表明它们具有氧化木质素等复杂分子的能力。
Wood-decay fungi are characterized by ligninolytic and hydrolytic enzymes that act through non-specific oxidation and hydrolytic reactions. The objective of this work was to evaluate the production of lignocellulolytic enzymes from collected fungi and to analyze their growth on lignocellulosic material. The study considered 18 species isolated from collections made in the state of Chiapas, Mexico, identified by taxonomic and molecular techniques, finding 11 different families. The growth rates of each isolate were obtained in culture media with African palm husk (PH), coffee husk (CH), pine sawdust (PS), and glucose as control, measuring daily growth with images analyzed in ImageJ software, finding the highest growth rate in the CH medium. The potency index (PI) of cellulase, xylanase, and manganese peroxidase (MnP) activities was determined, as well as the quantification of lignin peroxidase (LiP), with the strains Phlebiopsis flavidoalba TecNM-ITTG L20-19 and Phanerochaete sordida TecNM-ITTG L32-1-19 being the ones with the highest PI of hydrolase activities with 2.01 and 1.83 cellulase PI and 1.95 and 2.24 xylanase PI, respectively, while Phlebiopsis flavidoalba TecNM-ITTG L20-19 and Trametes sanguinea TecNM-ITTG L14-19 with 7115 U/L LiP activity had the highest oxidase activities, indicating their ability to oxidize complex molecules such as lignin.
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DOI：
10.3390/jof7040309
发表时间：
2021-04-16
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Alshammari N;Ameen F;AlKahtani MDF;Stephenson S
通讯作者：
Stephenson S
影响因子：
4.4
作者：
Al-Zaban MI;AlHarbi MA;Mahmoud MA
通讯作者：
Mahmoud MA
影响因子：
1.4
作者：
Martinez, Sebastian;Nakasone, Karen K.;Bettucci, Lina
通讯作者：
Bettucci, Lina
影响因子：
--
作者：
Maldonado-Bustamante, S.R.;Mondaca-Fernández, I.;Balderas-Cortés, J.J.
通讯作者：
Balderas-Cortés, J.J.
影响因子：
5
作者：
Morales, Paulina;Caceres, Manuel;Vergara-Fernandez, Alberto
通讯作者：
Vergara-Fernandez, Alberto