Evaluation of three different decontamination techniques on biofilm formation, and on physical and chemical properties of resin composites.
Evaluation of three different decontamination techniques on biofilm formation, and on physical and chemical properties of resin composites.
复制标题
DOI：
10.1002/jbm.b.33907
复制
发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Ferracane JL
中科院分区：
文献类型：
--
作者：
André CB;Dos Santos A;Pfeifer CS;Giannini M;Girotto EM;Ferracane JL
本研究评估了三种不同的树脂复合材料灭菌/消毒技术对细菌生长和去污后表面改性的影响。采用紫外光、1%氯胺T和70%乙醇三种不同的技术对两种树脂复合材料进行灭菌/消毒。每种技术使用四种不同的时间来确定溶液或紫外线有效的最短时间。通过分析代谢活性、使用AlamarBlue™检测、细菌活力和变形链球菌生物膜的SEM图像来评估灭菌/消毒技术对细菌生长的影响。利用ATR/FTIR和SEM分析了加工后的表面变化。用1H-NMR分析用于去污的溶液（1%氯胺- t和70%乙醇），以确定在此过程中浸出的任何树脂化合物。一分钟的去污对所有测试的三种方法都是有效的。氯胺- t增加了树脂复合材料的表面孔隙率，在紫外线和70%乙醇的作用下没有观察到变化，但只有在70%乙醇的作用下，1H-NMR才能识别出浸出的单体。净化后的ATR/FTIR分析未发现材料的化学变化。未经清洗的氯胺- t增加了两种树脂复合材料的细菌活力，并增加了不含氟化物的树脂复合材料的细菌代谢。与其他去污方法相比，紫外线对暴露1分钟的树脂复合材料性能测试没有干扰。
This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans. The surface change, after the process, was analyzed with ATR/FTIR and SEM images. The solutions used for decontamination (1% chloramine-T and 70% ethanol) were analyzed with 1H-NMR to identify any resin compounds leached during the process. One minute of decontamination was efficient for all three methods tested. Chloramine-T increased the surface porosity on resin composites, no changes were observed for UV light and 70% ethanol, however, 1H-NMR identified leached monomers only when 70% ethanol was used. No chemical change of the materials was found under ATR/FTIR analyses after the decontamination process. Chloramine-T, with no previous wash, increased the bacterial viability for both resin composites and increased the bacterial metabolism for the resin composite without fluoride. UV light had no interference on the resin composites properties tested using 1 min of exposure compared to the other decontamination methods.
登录
查看更多内容
影响因子：
2.2
作者：
Park, J. W.;Song, C. W.;Ferracane, J. L.
通讯作者：
Ferracane, J. L.
影响因子：
4.4
作者：
LEE, SY;GREENER, EH;MUELLER, HJ
通讯作者：
MUELLER, HJ
影响因子：
5
作者：
Hannig, Christian;Duong, Sebastian;Attin, Thomas
通讯作者：
Attin, Thomas
影响因子：
1.9
作者：
Polydorou, Olga;Koenig, Armin;Kuemmerer, Klaus
通讯作者：
Kuemmerer, Klaus
影响因子：
4.4
作者：
Farrugia, Cher;Cassar, Glenn;Camilleri, Josette
通讯作者：
Camilleri, Josette