Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity.
Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity.
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DOI：
10.1038/s41396-020-00763-3
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Hughes GL
中科院分区：
文献类型：
--
作者：
Kozlova EV;Hegde S;Roundy CM;Golovko G;Saldaña MA;Hart CE;Anderson ER;Hornett EA;Khanipov K;Popov VL;Pimenova M;Zhou Y;Fovanov Y;Weaver SC;Routh AL;Heinz E;Hughes GL
微生物之间的相互作用如何决定蚊子肠道中微生物的复杂性尚不清楚。以前，我们发现，沙雷氏菌，肠道共生体，改变载体的能力，并正在考虑为载体控制，很少殖民埃及伊蚊，但在相同条件下饲养的致倦库蚊丰富。目的探讨沙雷氏菌属与山羊草属的不亲和性。埃及，我们的特点是两个不同的菌株粘质沙雷氏菌Cx。quinquefasciatus，并检查其感染Ae.埃及人。两种沙雷氏菌属菌株对Ae.埃及的感染，但当微生物组稳态被破坏时，沙雷氏菌属的流行率和滴度与其天然宿主中的感染相似。研究多种遗传多样的Ae。埃及菌株发现微生物对S.粘质虫很常见，然而，Ae的一条线。埃及人易受感染。耐药和敏感株系的微生物组分析表明肠杆菌科细菌和沙雷氏菌之间存在负相关性，并且在gnotobiotic系统中的实验性共感染重现了干扰表型。此外，我们观察到宿主行为的影响;沙雷氏菌暴露于Ae。埃及伊蚊破坏了它们的进食行为，这种表型也依赖于与它们的原生微生物群的相互作用。我们的工作突出了宿主-微生物相互作用的复杂性，并提供了微生物相互作用影响蚊子行为的证据。
How microbe–microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that, Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti yet was abundant in Culex quinquefasciatus reared under identical conditions. To investigate the incompatibility between Serratia and Ae. aegypti, we characterized two distinct strains of Serratia marcescens from Cx. quinquefasciatus and examined their ability to infect Ae. aegypti. Both Serratia strains poorly infected Ae. aegypti, but when microbiome homeostasis was disrupted, the prevalence and titers of Serratia were similar to the infection in its native host. Examination of multiple genetically diverse Ae. aegypti lines found microbial interference to S. marcescens was commonplace, however, one line of Ae. aegypti was susceptible to infection. Microbiome analysis of resistant and susceptible lines indicated an inverse correlation between Enterobacteriaceae bacteria and Serratia, and experimental co-infections in a gnotobiotic system recapitulated the interference phenotype. Furthermore, we observed an effect on host behavior; Serratia exposure to Ae. aegypti disrupted their feeding behavior, and this phenotype was also reliant on interactions with their native microbiota. Our work highlights the complexity of host–microbe interactions and provides evidence that microbial interactions influence mosquito behavior.
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影响因子：
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作者：
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影响因子：
6.4
作者：
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影响因子：
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Kanuka, Hirotaka