Investigating CRISPR spacer targets and their impact on genomic diversification of Streptococcus mutans.
Investigating CRISPR spacer targets and their impact on genomic diversification of Streptococcus mutans.
复制标题
研究CRISPR间隔区靶点及其对变形链球菌基因组多样性的影响。
DOI：
10.3389/fgene.2022.997341
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发表时间：
2022
影响因子：
3.7
通讯作者：
Shields, Robert C. C.
中科院分区：
文献类型：
--
作者：
Walker, Alejandro R. R.;Shields, Robert C. C.
关键词：
CRISPR-Cas 是一种限制移动 DNA 元件获取的细菌免疫系统。这些系统通过编码 CRISPR 间隔区来提供针对外来 DNA 的免疫力，当 DNA 重新进入细胞时，这些间隔区有助于靶向 DNA。这样，CRISPR间隔区是宿主微生物遇到的外源DNA的一种分子磁带记录器。在这里，我们从 300 多个变形链球菌基因组中提取了约 8,000 个 CRISPR 间隔区。噬菌体 DNA 是变形链球菌间隔区的主要目标。变形链球菌菌株还产生了针对移动 DNA 元件（例如质粒以及整合和接合元件）的免疫力。尽管自我靶向与真正的种内或种间靶向的相对贡献需要进一步研究，但也可能对细菌 DNA 产生相当大的免疫力。虽然有明确的证据表明这些系统已经获得了针对外源 DNA 的免疫力，但对物种水平上的水平基因转移 (HGT) 限制的影响似乎很小。当比较具有低或高 CRISPR 间隔区负载的变形链球菌菌株时，对基因组大小、GC 含量和全基因组的“开放性”影响很小或没有影响。总之，虽然有证据表明 CRISPR 间隔区针对自身和外源 DNA 获得，但 CRISPR-Cas 并不能成为变形链球菌辅助基因组扩展的障碍。
CRISPR-Cas is a bacterial immune system that restricts the acquisition of mobile DNA elements. These systems provide immunity against foreign DNA by encoding CRISPR spacers that help target DNA if it re-enters the cell. In this way, CRISPR spacers are a type of molecular tape recorder of foreign DNA encountered by the host microorganism. Here, we extracted ∼8,000 CRISPR spacers from a collection of over three hundred Streptococcus mutans genomes. Phage DNA is a major target of S. mutans spacers. S. mutans strains have also generated immunity against mobile DNA elements such as plasmids and integrative and conjugative elements. There may also be considerable immunity generated against bacterial DNA, although the relative contribution of self-targeting versus bona fide intra- or inter-species targeting needs to be investigated further. While there was clear evidence that these systems have acquired immunity against foreign DNA, there appeared to be minimal impact on horizontal gene transfer (HGT) constraints on a species-level. There was little or no impact on genome size, GC content and ‘openness’ of the pangenome when comparing between S. mutans strains with low or high CRISPR spacer loads. In summary, while there is evidence of CRISPR spacer acquisition against self and foreign DNA, CRISPR-Cas does not act as a barrier on the expansion of the S. mutans accessory genome.
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影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
DOI：
10.1038/ismej.2015.20
发表时间：
2015-09
期刊：
The ISME journal
影响因子：
--
作者：
Gophna U;Kristensen DM;Wolf YI;Popa O;Drevet C;Koonin EV
通讯作者：
Koonin EV
影响因子：
6.4
作者：
Khan R;Rukke HV;Høvik H;Åmdal HA;Chen T;Morrison DA;Petersen FC
通讯作者：
Petersen FC
影响因子：
2.6
作者：
Meng, Peiqi;Lu, Chang;Chen, Feng
通讯作者：
Chen, Feng
影响因子：
5.8
作者：
Page, Andrew J.;Cummins, Carla A.;Parkhill, Julian
通讯作者：
Parkhill, Julian