Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells.
Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells.
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DOI：
10.3892/or.2013.2635
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发表时间：
2013-10
期刊：
影响因子：
4.2
通讯作者：
Chai YG
中科院分区：
文献类型：
--
作者：
Lee HT;Choi MR;Doh MS;Jung KH;Chai YG
化疗是几种癌症(包括前列腺癌)中用于抑制癌细胞增殖的治疗策略之一。虽然单胺氧化酶(MAO)抑制剂、植物雌激素和抗氧化剂在化疗中的应用已经得到了系统的研究，但它们对癌细胞生长的影响仍不完全清楚。本研究旨在探讨MAO抑制剂帕吉林和三羟环丙胺对人前列腺癌(LNCaP-LN3)细胞存活的影响。用帕吉林或三羟环丙胺处理LNCaP-LN3细胞后，检测细胞增殖、细胞周期、细胞凋亡及凋亡相关基因的表达水平。暴露于pargyline的细胞以剂量和时间依赖的方式抑制增殖，而三羟环丙胺处理的细胞则表现出相反的结果。与对照组和三羟环丙胺处理的细胞相比，pargyline处理显著诱导细胞周期停滞于G1期。此外，pargyline通过促进细胞凋亡而增加细胞死亡率，而三羟环丙胺对LNCaP-LN3细胞无影响。根据我们的结果，我们认为帕吉林治疗前列腺癌比三羟环丙胺更有效。
Chemotherapy is one of the therapeutic strategies that has been used for the inhibition of cancer cell proliferation in several types of cancer, including prostate cancer. Although monoamine oxidase (MAO) inhibitors, phytoestrogen and antioxidants used in chemotherapy have been systematically studied, their effects on cancer cell growth remain to be fully understood. The purpose of this study was to investigate the effects of the MAO inhibitors, pargyline and tranylcypromine on cell survival in human prostate carcinoma (LNCaP-LN3) cells. After treating LNCaP-LN3 cells with pargyline or tranylcypromine, we examined cell proliferation, cell cycle pattern, apoptosis and the expression levels of apoptosis-related genes. The proliferation of cells exposed to pargyline decreased in a dose- and time-dependent manner, while tranylcypromine-treated cells showed the opposite results. Treatment with pargyline significantly induced cell cycle arrest at the G1 phase compared to the control and tranylcypromine-treated cells. In addition, pargyline induced an increase in the cell death rate by promoting apoptosis; however, tranylcypromine had no effect on LNCaP-LN3 cells. Based on our results, we suggest that pargyline is more powerful than tranylcypromine for the treatment of human prostate cancer.
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