Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India.
Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India.
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DOI：
10.1186/2193-1801-1-73
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Kotasthane AS
中科院分区：
文献类型：
--
作者：
Agrawal T;Kotasthane AS
几丁质是自然界中仅次于纤维素的第二大聚合物，在真菌细胞壁中起着重要作用。木霉菌作为一种几丁质酶的产生菌，已成为真菌病害生物防治的重要手段。本文提出了一种简单、灵敏的方法，以胶体几丁质为唯一碳源，添加溴甲酚紫（pH指示染料）作为基本培养基，用于测定大种群木霉几丁质酶活性。用pH指示染料（溴甲酚紫，BCP）作为可溶性底物测定固体培养基上几丁质酶的活性，是一种灵敏、简便、重复性好的半定量酶扩散板法，也是一种经济的测定几丁质酶活性的方法。来源于丝核菌细胞壁的胶体几丁质和作为碳源的商业几丁质也允许选择和比较木霉中几丁质分解和外切几丁质酶活性。在丝核菌细胞壁和商业几丁质衍生的胶体几丁质补充培养液中，N-乙酰基-β-D-葡萄糖胺（那牙）的释放范围分别为37.67 - 174.33mg/ml和37.67 - 327.67mg/ml，对硝基苯酚（pNP）的释放范围分别为0.17 - 35.78 × 10- 3U/ml和0.62 - 32.6 × 10- 3U/ml。
Chitin is the second most abundant polymer in nature after cellulose and plays a major role in fungal cell walls. As a producer of variety of chitinase enzymes Trichoderma has become an important means of biological control of fungal diseases. A simple and sensitive method based on the use of basal medium with colloidal chitin as sole carbon source supplemented with Bromo cresol purple (pH indicator dye) is proposed to evaluate large populations of Trichoderma for chitinase activity. The soluble substrate with pH indicator dye (Bromo cresol purple, BCP) for the assay of chitinase activity on solid media is sensitive, easy, reproducible semi-quantitative enzyme diffusion plate assay and economic option to determine chitinases. Colloidal chitin derived from Rhizoctonia cell wall and commercial chitin included as a carbon source in broth also allowed selection and comparison of chitinolytic and exochitinase activity in Trichoderma spectrophotometrically. Released N-acetyl-β--D-glucosamine (NAGA) ranged from 37.67 to 174.33 mg/ml and 37.67 to 327.67 mg/ml and p-nitrophenol (pNP) ranged from 0.17 to 35.78 X 10-3 U/ml and 0.62 to 32.6 X 10-3 U/ml) respectively with Rhizoctonia cell wall and commercial chitin derived colloidal chitin supplemented broth.
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影响因子：
5.5
作者：
Calistru, C;McLean, M;Berjak, P
通讯作者：
Berjak, P
影响因子：
3.2
作者：
LORITO, M;HAYES, CK;HARMAN, GE
通讯作者：
HARMAN, GE
DOI：
10.1080/03601230701771164
发表时间：
2008-01-01
影响因子：
2
作者：
Goswami, Jaideep;Pandey, Rajesh Kumar;Goswami, B. K.
通讯作者：
Goswami, B. K.
影响因子：
2.2
作者：
MCCREATH, KJ;GOODAY, GW
通讯作者：
GOODAY, GW
影响因子：
2.8
作者：
MONREAL, J;REESE, ET
通讯作者：
REESE, ET