Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
复制标题
DOI：
10.1371/journal.pone.0100430
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Templeton SP
中科院分区：
文献类型：
--
作者：
Amarsaikhan N;O'Dea EM;Tsoggerel A;Owegi H;Gillenwater J;Templeton SP
无处不在的真菌病原体烟曲霉是易感个体的过敏致敏和侵袭性疾病的中介。临床和环境分离株之间的显著遗传和表型变异是烟曲霉介导疾病宿主-病原体研究的重要考虑因素。我们观察到，与其他临床和环境分离菌株相比，单一环境分离菌株Af5517的径向生长、发芽率和建立菌落生长的能力都有所下降。Af5517菌丝直径、细胞壁β-葡聚糖和几丁质含量均增加，其中几丁质含量增加最为显著。与临床分离株Af293相比，中性粒细胞减少的af5517感染小鼠的发病率、死亡率、肺部真菌负荷和组织病理学均有所降低。我们的研究结果支持了先前的研究结果，即体外生长速度与体内毒力之间存在相关性，我们提出细胞壁组成的变化可能有助于这种表型。
The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
登录
查看更多内容
Dectin-1介导β-葡聚糖的生物学作用。
DOI：
10.1084/jem.20021890
发表时间：
2003-05-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Brown GD;Herre J;Williams DL;Willment JA;Marshall AS;Gordon S
通讯作者：
Gordon S
影响因子：
2.2
作者：
Balajee, S. Arunmozhi;de Valk, Hanneke A.;Klaassen, Corne H. W.
通讯作者：
Klaassen, Corne H. W.
影响因子：
5.4
作者：
Lenardon, Megan D.;Munro, Carol A.;Gow, Neil A. R.
通讯作者：
Gow, Neil A. R.
影响因子：
5.8
作者：
Chai, Louis Y. A.;Vonk, Alieke G.;Netea, Mihai G.
通讯作者：
Netea, Mihai G.
影响因子：
4.9
作者：
Bowman, JC;Abruzzo, GK;Douglas, CM
通讯作者：
Douglas, CM