DNA barcodes for the fishes of the Narmada, one of India's longest rivers.
DNA barcodes for the fishes of the Narmada, one of India's longest rivers.
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DOI：
10.1371/journal.pone.0101460
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Haymer D
中科院分区：
文献类型：
--
作者：
Khedkar GD;Jamdade R;Naik S;David L;Haymer D
本研究描述了印度纳尔默达河鱼类的物种多样性。从整个流域的17个采样点共采集鱼类标本820份。根据形态特征将鱼类分类为90个可能的种之一，然后使用COI基因序列作为辅助鉴定方法，使用DNA条形码进行鉴定。共获得314条不同的COI序列，经鉴定，共隶属于10目34科63属85种。这项研究的结果包括确定5个假定的神秘或兄弟物种和43种以前不知道从纳尔默达河流域。五种是印度特有的，三种是以前没有报道过在纳尔默达河发生的引进物种。相反，43种以前报道发生在Narmada没有found.Genetic多样性和距离值内的属，科和目的所有物种使用Kimura的2参数距离模型，然后构建的邻居连接树。在NJ树中生成的高分辨率聚类有助于将对应于其属和科的物种分组，这与自动条形码缺口发现生物信息学平台生成的值相确认。这有助于确定一个阈值的歧视从纳尔默达河的物种边界。这项研究提供了一个重要的验证使用DNA条形码序列监测物种多样性和复杂的生态系统，如纳尔默达河的变化。
This study describes the species diversity of fishes of the Narmada River in India. A total of 820 fish specimens were collected from 17 sampling locations across the whole river basin. Fish were taxonomically classified into one of 90 possible species based on morphological characters, and then DNA barcoding was employed using COI gene sequences as a supplemental identification method. A total of 314 different COI sequences were generated, and specimens were confirmed to belong to 85 species representing 63 genera, 34 families and 10 orders. Findings of this study include the identification of five putative cryptic or sibling species and 43 species not previously known from the Narmada River basin. Five species are endemic to India and three are introduced species that had not been previously reported to occur in the Narmada River. Conversely, 43 species previously reported to occur in the Narmada were not found. Genetic diversity and distance values were generated for all of the species within genera, families and orders using Kimura’s 2 parameter distance model followed by the construction of a Neighbor Joining tree. High resolution clusters generated in NJ trees aided the groupings of species corresponding to their genera and families which are in confirmation to the values generated by Automatic Barcode Gap Discovery bioinformatics platform. This aided to decide a threshold value for the discrimination of species boundary from the Narmada River. This study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within complex ecosystems such as the Narmada River.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
9.8
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
通讯作者：
Francis CM
影响因子：
2.4
作者：
Holland, BS;Dawson, MN;Hofmann, DK
通讯作者：
Hofmann, DK
影响因子：
3.4
作者：
Dépraz A;Hausser J;Pfenninger M
通讯作者：
Pfenninger M
DOI：
10.1098/rspb.2006.3718
发表时间：
2007-01-22
影响因子：
4.7
作者：
Gomez, Africa;Wright, Peter J.;Hughes, Roger N.
通讯作者：
Hughes, Roger N.