Functional hierarchy and reversibility within the murine spermatogenic stem cell compartment.
Functional hierarchy and reversibility within the murine spermatogenic stem cell compartment.
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DOI：
10.1126/science.1182868
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发表时间：
2010-04-02
期刊：
影响因子：
--
通讯作者：
Yoshida S
中科院分区：
文献类型：
--
作者：
Nakagawa T;Sharma M;Nabeshima Y;Braun RE;Yoshida S
干细胞通过平衡自我更新和分化来支持组织维持。在小鼠中，人们认为单个精原细胞的同质干细胞群支持精子发生，伴随着长度增加的连接细胞(囊)的形成的分化是线性的和不可逆的。我们使用谱系分析和活体成像对这一模型进行了评估，发现这个假定的干细胞群体并不是同质的。相反，支持稳定精子发生的干细胞库包含在单个精原细胞的一个亚群中。此外，囊肿并不致力于分化，似乎通过碎裂来恢复干细胞潜能。最后，在损伤后的再生过程中，单个精原种群的命运发生了戏剧性的变化。因此，从干细胞到分化有多种可逆的途径，这也可能发生在其他系统中。
Stem cells support tissue maintenance by balancing self-renewal and differentiation. In mice, it is believed that a homogeneous stem cell population of single spermatogonia supports spermatogenesis, and that differentiation, which is accompanied by the formation of connected cells (cysts) of increasing length, is linear and nonreversible. We evaluated this model using lineage-analysis and live-imaging and found that this putative stem cell population is not homogeneous. Instead, the stem cell pool that supports steady-state spermatogenesis is contained within a subpopulation of single spermatogonia. Also, cysts are not committed to differentiation and appear to recover stem cell potential by fragmentation. Lastly, the fate of individual spermatogonial populations was dramatically altered during regeneration following damage. Thus, there are multiple and reversible paths from stem cell to differentiation, which may also occur in other systems.
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