Laser spectroscopic technique for direct identification of a single virus I: FASTER CARS.
Laser spectroscopic technique for direct identification of a single virus I: FASTER CARS.
复制标题
DOI：
10.1073/pnas.2013169117
复制
发表时间：
2020-11-10
影响因子：
11.1
通讯作者：
Scully MO
中科院分区：
文献类型：
--
作者：
Deckert V;Deckert-Gaudig T;Cialla-May D;Popp J;Zell R;Deinhard-Emmer S;Sokolov AV;Yi Z;Scully MO
病毒的表面特征对决定其生殖力非常重要。例如，严重急性呼吸综合征冠状病毒2 （SARS- cov -2）的刺突蛋白与宿主细胞的ACE2受体位点结合的亲和力比原始SARS病毒强得多。因此，了解病毒粒子的表面结构显然很重要。为此，本文将原子力显微镜的空间分辨率与相干拉曼光谱的光谱分辨率相结合。利用飞秒自适应光谱技术对相干反斯托克斯拉曼散射（FAST CARS）进行尖端增强显微镜的结合，提高了分辨率（FASTER CARS），使我们能够以纳米分辨率和化学特异性绘制单个病毒颗粒。从著名的1918年H1N1流感到目前的COVID-19大流行，改进病毒检测技术的必要性是显而易见的。本文的目的是表明在临床样品材料中快速可靠地鉴定单个病毒颗粒近在咫尺。首先，我们的团队开发了基于模块化原子力显微镜（AFM）的病毒粒子识别技术。此外，通过相干反斯托克斯拉曼散射提高分辨率的飞秒自适应光谱技术（FASTER CARS）使用尖端增强技术显着提高了灵敏度[M]。O.史考利等人，《联邦诉讼法》。学会科学。[j].美国，1999,10994 - 11001（2002）。
Surface features of a virus are very important in determining its virility. For example, the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds to the ACE2 receptor site of the host cell with a much stronger affinity than did the original SARS virus. Thus, it is clearly important to understand the virion surface structure. To that end, the present paper combines the spatial resolution of atomic force microscopy and the spectral resolution of coherent Raman spectroscopy. This combination of tip-enhanced microscopy using femtosecond adaptive spectroscopic techniques for coherent anti-Stokes Raman scattering (FAST CARS) with enhanced resolution (FASTER CARS) allows us to map a single virus particle with nanometer resolution and chemical specificity. From the famous 1918 H1N1 influenza to the present COVID-19 pandemic, the need for improved viral detection techniques is all too apparent. The aim of the present paper is to show that identification of individual virus particles in clinical sample materials quickly and reliably is near at hand. First of all, our team has developed techniques for identification of virions based on a modular atomic force microscopy (AFM). Furthermore, femtosecond adaptive spectroscopic techniques with enhanced resolution via coherent anti-Stokes Raman scattering (FASTER CARS) using tip-enhanced techniques markedly improves the sensitivity [M. O. Scully, et al., Proc. Natl. Acad. Sci. U.S.A. 99, 10994–11001 (2002)].
登录
查看更多内容
影响因子：
2.5
作者：
Cialla, Dana;Deckert-Gaudig, Tanja;Popp, Juergen
通讯作者：
Popp, Juergen
影响因子：
15
作者：
He, Zhe;Han, Zehua;Scully, Marlan O.
通讯作者：
Scully, Marlan O.
影响因子：
56.9
作者：
Pestov, Dmitry;Murawski, Robert K.;Scully, Marlan O.
通讯作者：
Scully, Marlan O.
影响因子：
4.6
作者：
Deckert-Gaudig T;Kurouski D;Hedegaard MA;Singh P;Lednev IK;Deckert V
通讯作者：
Deckert V
影响因子：
3.7
作者：
Hermann, Peter;Fabian, Heinz;Hermelink, Antje
通讯作者：
Hermelink, Antje