Development of a large volume concentration method for recovery of coronavirus from wastewater.
Development of a large volume concentration method for recovery of coronavirus from wastewater.
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DOI：
10.1016/j.scitotenv.2021.145727
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发表时间：
2021-06-20
期刊：
影响因子：
--
通讯作者：
Oshima K
中科院分区：
文献类型：
--
作者：
McMinn BR;Korajkic A;Kelleher J;Herrmann MP;Pemberton AC;Ahmed W;Villegas EN;Oshima K
废水中 2 型严重急性呼吸道冠状病毒 (SARS CoV 2) RNA 的水平可以作为监测社区内 2019 年冠状病毒病 (COVID-19) 的有效手段。然而，目前用于检测废水中 SARS CoV 2 RNA 的方法在处理足够量的源材料的能力方面受到限制，从而抑制了我们评估病毒载量的能力。通常，使用初级和二级浓缩从大量液体中浓缩病毒。在这里，我们评估了用于初级浓缩的死端中空纤维超滤器 (D-HFUF)，然后评估了用于对 2 L 初级处理废水进行二级浓缩的 CP Select™。研究了对每个浓缩程序的各种修改，以最佳地从废水中回收接种的 OC43（β 冠状病毒）。在初级浓缩过程中，D-HFUF 从 2 L 废水中回收了 69 ± 18% (n = 29) 的加标 OC43。对于二次浓缩，使用废水应用设置的 CP Select™ 系统能够在 25 分钟内处理 100 mL 体积的初级过滤器洗脱液。事实证明，从过滤器洗脱液中回收 OC43 时，手动注射器洗脱明显优于 CP Select™ 洗脱 (p = 0.0299)，分别为 48 ± 2% 和 31 ± 3%。对于完整的方法（初级和二级浓缩相结合），D-HFUF 和 CP 选择/注射器洗脱通过 (n = 8) 重复过滤器实现了加标 OC43 的总体回收率 22 ± 4%。鉴于缺乏可用的标准化方法，再加上依靠病毒 RNA 进行 SARS CoV 2 废水监测的固有局限性，在解释这些数据以估计社区感染率时，必须承认这些挑战。然而，开发能够大幅增加样本量的方法可能会允许报告用于废水监测的可量化病毒数据，为公共卫生官员提供更好地估计社区感染率所需的信息。
Levels of severe acute respiratory coronavirus type 2 (SARS CoV 2) RNA in wastewater could act as an effective means to monitor coronavirus disease 2019 (COVID-19) within communities. However, current methods used to detect SARS CoV 2 RNA in wastewater are limited in their ability to process sufficient volumes of source material, inhibiting our ability to assess viral load. Typically, viruses are concentrated from large liquid volumes using two stage concentration, primary and secondary. Here, we evaluated a dead-end hollow fiber ultrafilter (D-HFUF) for primary concentration, followed by the CP Select™ for secondary concentration from 2 L volumes of primary treated wastewater. Various amendments to each concentration procedure were investigated to optimally recover seeded OC43 (betacoronavirus) from wastewater. During primary concentration, the D-HFUF recovered 69 ± 18% (n = 29) of spiked OC43 from 2 L of wastewater. For secondary concentration, the CP Select™ system using the Wastewater Application settings was capable of processing 100 mL volumes of primary filter eluates in <25 min. A hand-driven syringe elution proved to be significantly superior (p = 0.0299) to the CP Select™ elution for recovering OC43 from filter eluates, 48 ± 2% compared to 31 ± 3%, respectively. For the complete method (primary and secondary concentration combined), the D-HFUF and CP select/syringe elution achieved overall 22 ± 4% recovery of spiked OC43 through (n = 8) replicate filters. Given the lack of available standardized methodology confounded by the inherent limitations of relying on viral RNA for wastewater surveillance of SARS CoV 2, it is important to acknowledge these challenges when interpreting this data to estimate community infection rates. However, the development of methods that can substantially increase sample volumes will likely allow for reporting of quantifiable viral data for wastewater surveillance, equipping public health officials with information necessary to better estimate community infection rates.
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发表时间：
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期刊：
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