An overview of tissue and whole organ decellularization processes.
An overview of tissue and whole organ decellularization processes.
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DOI：
10.1016/j.biomaterials.2011.01.057
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发表时间：
2011-04
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Crapo, Peter M.;Gilbert, Thomas W.;Badylak, Stephen F.
由细胞外基质（ECM）组成的生物支架材料通常通过涉及组织或器官脱细胞的过程获得。保留细胞外基质复杂的成分和三维超微结构是非常理想的，但人们认识到所有脱细胞方法都会导致结构破坏以及表面结构和成分的潜在损失。物理方法以及化学和生物制剂联合使用来裂解细胞，然后冲洗以去除细胞残余物。有效的脱细胞方法由诸如组织密度和组织结构、最终产品所需的几何和生物学特性以及目标临床应用等因素决定。通过在处理过程中对所使用的试剂和技术做出明智的决策，可以优化在保留细胞外基质完整性和生物活性的情况下进行组织脱细胞。本文综述了脱细胞方法、它们对所得细胞外基质结构和成分的影响，以及最近描述的用于全器官脱细胞技术的灌注技术。
Biologic scaffold materials composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Preservation of the complex composition and three-dimensional ultrastructure of the ECM is highly desirable but it is recognized that all methods of decellularization result in disruption of the architecture and potential loss of surface structure and composition. Physical methods and chemical and biologic agents are used in combination to lyse cells, followed by rinsing to remove cell remnants. Effective decellularization methodology is dictated by factors such as tissue density and organization, geometric and biologic properties desired for the end product, and the targeted clinical application. Tissue decellularization with preservation of ECM integrity and bioactivity can be optimized by making educated decisions regarding the agents and techniques utilized during processing. An overview of decellularization methods, their effect upon resulting ECM structure and composition, and recently described perfusion techniques for whole organ decellularization techniques are presented herein.
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影响因子：
3.6
作者：
Borschel, GH;Dennis, RG;Kuzon, WM
通讯作者：
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影响因子：
14
作者：
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影响因子：
14
作者：
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通讯作者：
Badylak, Stephen F.
影响因子：
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通讯作者：
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影响因子：
2.4
作者：
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