Insulin-producing cells regulate the sexual receptivity through the painless TRP channel in Drosophila virgin females.
Insulin-producing cells regulate the sexual receptivity through the painless TRP channel in Drosophila virgin females.
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DOI：
10.1371/journal.pone.0088175
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kitamoto T
中科院分区：
文献类型：
--
作者：
Sakai T;Watanabe K;Ohashi H;Sato S;Inami S;Shimada N;Kitamoto T
在许多动物物种中，雌性在评估潜在的交配伴侣方面处于领先地位。处女雌性接受或拒绝求爱雄性的决定是交配成功的最关键步骤之一。然而，在果蝇中，雌性感受性的分子和神经机制仍然知之甚少，特别是对于处女雌性。果蝇无痛（pain）基因编码一个瞬时受体电位（TRP）离子通道。我们以前证明，疼痛的突变显着增强处女女性的性感受性和painGAL 4阳性神经元的疼痛表达是必要的和足够的疼痛介导的调节处女感受性。在成年女性大脑中的painGAL4阳性神经元中，我们发现胰岛素产生细胞（IPC）是大脑间部的一个神经元亚群，在处女女性中通过Pain TRP通道调节性感受性是必不可少的。IPC特异性敲低疼痛表达或IPC消融强烈增强女性性感受性，如在疼痛突变女性中观察到的。当疼痛的表达或神经元的活动被有条件地抑制在成人IPC，女性性感受性同样增强。此外，疼痛突变和条件性敲低的疼痛表达的IPC抑郁症女性拒绝求爱的男性行为。综上所述，我们的研究结果表明，在IPC的疼痛TRP通道起着重要的作用，在控制果蝇处女雌性的性接受性，积极调节女性在求爱期间的拒绝行为。
In a variety of animal species, females hold a leading position in evaluating potential mating partners. The decision of virgin females to accept or reject a courting male is one of the most critical steps for mating success. In the fruitfly Drosophila melanogaster, however, the molecular and neuronal mechanisms underlying female receptivity are still poorly understood, particularly for virgin females. The Drosophila painless (pain) gene encodes a transient receptor potential (TRP) ion channel. We previously demonstrated that mutations in pain significantly enhance the sexual receptivity of virgin females and that pain expression in painGAL4-positive neurons is necessary and sufficient for pain-mediated regulation of the virgin receptivity. Among the painGAL4-positive neurons in the adult female brain, here we have found that insulin-producing cells (IPCs), a neuronal subset in the pars intercerebralis, are essential in virgin females for the regulation of sexual receptivity through Pain TRP channels. IPC-specific knockdown of pain expression or IPC ablation strongly enhanced female sexual receptivity as was observed in pain mutant females. When pain expression or neuronal activity was conditionally suppressed in adult IPCs, female sexual receptivity was similarly enhanced. Furthermore, both pain mutations and the conditional knockdown of pain expression in IPCs depressed female rejection behaviors toward courting males. Taken together, our results indicate that the Pain TRP channel in IPCs plays an important role in controlling the sexual receptivity of Drosophila virgin females by positively regulating female rejection behaviors during courtship.
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