A comprehensive expression profile of microRNAs in porcine pituitary.
A comprehensive expression profile of microRNAs in porcine pituitary.
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猪垂体中 MicroRNA 的综合表达谱
DOI：
10.1371/journal.pone.0024883
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Yuan L
中科院分区：
文献类型：
--
作者：
Li H;Xi Q;Xiong Y;Cheng X;Qi Q;Yang L;Shu G;Wang S;Wang L;Gao P;Zhu X;Jiang Q;Zhang Y;Yuan L
MicroRNAs(MiRNAs)是一类丰富的小RNA，调节大多数基因的表达。MiRNAs在脑下垂体这个内分泌器官中扮演着重要的角色，但目前还不清楚miRNAs在脑下垂体组织的发育过程中起到了什么作用，也不知道它对脑垂体功能有多大的贡献。通过应用微阵列分析和SolexA测序相结合的方法，我们在猪脑垂体中总共检测到450个miRNAs。RT-PCR验证表明，所获得的数据具有很高的置信度。根据目前的miRBase版本17.0，检测到的miRNAs包括169个已知的猪miRNAs，163个尚未在猪中发现的保守miRNAs，以及12个尚未在任何物种中鉴定的潜在新miRNAs，其中3个被Northern印迹法揭示。脑垂体中可能含有约80.17%属于该动物的miRNA型。用京都基因和基因组百科全书(KEGG)对10个高表达的miRNAs进行分析表明，浓缩的miRNAs不仅参与器官的发育，而且参与参与器官功能的各种细胞间和细胞内过程或途径。我们已经揭示了大量的猪miRNAs的存在以及一些潜在的新的miRNAs，并首次建立了一个全面的脑垂体miRNA表达谱。脑下垂体出人意料地含有多种miRNA类型，miRNA的作用参与了器官发育和功能的重要过程。
MicroRNAs (miRNAs) are an abundant class of small RNAs that regulate expressions of most genes. miRNAs play important roles in the pituitary, the “master” endocrine organ.However, we still don't know which role miRNAs play in the development of pituitary tissue or how much they contribute to the pituitary function. By applying a combination of microarray analysis and Solexa sequencing, we detected a total of 450 miRNAs in the porcine pituitary. Verification with RT-PCR showed a high degree of confidence for the obtained data. According to the current miRBase release17.0, the detected miRNAs included 169 known porcine miRNAs, 163 conserved miRNAs not yet identified in the pig, and 12 potentially new miRNAs not yet identified in any species, three of which were revealed using Northern blot. The pituitary might contain about 80.17% miRNA types belonging to the animal. Analysis of 10 highly expressed miRNAs with the Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the enriched miRNAs were involved not only in the development of the organ but also in a variety of inter-cell and inner cell processes or pathways that are involved in the function of the organ. We have revealed the existence of a large number of porcine miRNAs as well as some potentially new miRNAs and established for the first time a comprehensive miRNA expression profile of the pituitary. The pituitary gland contains unexpectedly many miRNA types and miRNA actions are involved in important processes for both the development and function of the organ.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
3.3
作者：
Harding, Christopher J.;Kunz, Sebastian;Heiz, Ulrich
通讯作者：
Heiz, Ulrich
影响因子：
56.9
作者：
Farh, KKH;Grimson, A;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
5.6
作者：
Bottoni, A;Piccin, D;Uberti, ECD
通讯作者：
Uberti, ECD