Yolk syncytial layer formation is a failure of cytokinesis mediated by Rock1 function in the early zebrafish embryo.
Yolk syncytial layer formation is a failure of cytokinesis mediated by Rock1 function in the early zebrafish embryo.
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DOI：
10.1242/bio.20121636
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发表时间：
2012-08-15
期刊：
影响因子：
2.4
通讯作者：
Korzh V
中科院分区：
文献类型：
--
作者：
Chu LT;Fong SH;Kondrychyn I;Loh SL;Ye Z;Korzh V
卵黄合胞体层（YSL）在斑马鱼发育过程中起着重要的作用。然而，人们对这种重要的胚外结构形成的细胞和分子机制知之甚少。在这里，我们证明了通过延时共聚焦显微镜的转基因株系表达膜靶向的绿色荧光蛋白，YSL的形式作为在第十次有丝分裂的子核之间的胞质分裂和先前存在的边缘细胞膜的回归的情况下的结果，从而转换成合胞体的胚盘的前边缘。我们发现，破坏细胞骨架的组成部分诱导形成一个扩大的YSL，并确定Rock1作为调节细胞骨架的动力学，导致YSL的形成。我们的研究结果表明，YSL的形式作为一个结果，控制胞质分裂失败的边缘卵裂球，和Rock1功能是必要的，这一过程的发生。揭示斑马鱼YSL形成的细胞和分子机制为其他组织以及病理条件下的合胞体发育提供了重要的见解。
The yolk syncytial layer (YSL) performs multiple critical roles during zebrafish development. However, little is known about the cellular and molecular mechanisms that underlie the formation of this important extraembryonic structure. Here, we demonstrate by timelapse confocal microscopy of a transgenic line expressing membrane-targeted GFP that the YSL forms as a result of the absence of cytokinesis between daughter nuclei at the tenth mitotic division and the regression of pre-existing marginal cell membranes, thus converting the former margin of the blastoderm into a syncytium. We show that disruption of components of the cytoskeleton induces the formation of an expanded YSL, and identify Rock1 as the regulator of cytoskeletal dynamics that lead to YSL formation. Our results suggest that the YSL forms as a result of controlled cytokinesis failure in the marginal blastomeres, and Rock1 function is necessary for this process to occur. Uncovering the cellular and molecular mechanisms underlying zebrafish YSL formation offers significant insight into syncytial development in other tissues as well as in pathological conditions.
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影响因子：
2.7
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