Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume
Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume
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D 型细胞周期蛋白基因家族的全基因组分析揭示了木本植物梅的差异表达模式和茎发育
DOI：
10.3390/f10020147
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发表时间：
2019-02
期刊：
影响因子：
2.9
通讯作者：
Qixiang Zhang
中科院分区：
文献类型：
--
作者：
Tangchun Zheng;Xiaokang Zhuo;Lulu Li;Jia Wang;Tangren Cheng;Qixiang Zhang
细胞周期蛋白是一类重要的细胞分裂调节因子，在植物生长发育中起着极其重要的作用。D型细胞周期蛋白（CYCD）是G1期的限速成分。在植物中，CYCD基因的研究主要集中在草本植物，而在多年生木本植物，尤其是观赏植物中的研究还很少。在这里，12梅CYCD（PmCYCD）基因的鉴定和特点。PmCYCD是根据拟南芥和水稻中的直向同源物命名的。各亚群CYCD的基因结构和保守结构域与它们在A. Thaliana和O. sativa.然而，PmCYCD在根、茎、叶、芽和果实器官中表现出不同的组织特异性表达模式。qRT-PCR结果表明，除PmCYCD 5;2和PmCYCD 7;1外，其余PmCYCD主要在叶芽、芽和茎中表达。此外，还分析了外源激素NAA、6-BA、GA 3、阿坝和蔗糖处理下PmCYCD基因在根中的转录水平。有趣的是，虽然PmCYCD是由蔗糖诱导的，但PmCYCD亚组之间的基因诱导程度不同。PmCYCD 1;2的诱导激素依赖于蔗糖的存在。NAA对PmCYCD 3;1的诱导有促进作用，糖和激素配合使用时诱导作用更强。综上所述，我们的研究表明，PmCYCDs在植物茎发育的功能，并提供了一个基础，选择细胞周期蛋白基因家族的成员作为候选基因的观赏植物育种。
Cyclins, a prominent class of cell division regulators, play an extremely important role in plant growth and development. D-type cyclins (CYCDs) are the rate-limiting components of the G1 phase. In plants, studies of CYCDs are mainly concerned with herbaceous plants, yet little information is available about these genes in perennial woody plants, especially ornamental plants. Here, twelve Prunus mume CYCD (PmCYCDs) genes are identified and characterized. The PmCYCDs were named on the basis of orthologues in Arabidopsis thaliana and Oryza sativa. Gene structure and conserved domains of each subgroup CYCDs was similar to that of their orthologues in A. thaliana and O. sativa. However, PmCYCDs exhibited different tissue-specific expression patterns in root, stem, leaf, bud, and fruit organs. The results of qRT-PCR showed that all PmCYCDs, except PmCYCD5;2 and PmCYCD7;1, were primarily highly expressed in leaf buds, shoots, and stems. In addition, the transcript levels of PmCYCD genes were analyzed in roots under different treatments, including exogenous applications of NAA, 6-BA, GA3, ABA, and sucrose. Interestingly, although PmCYCDs were induced by sucrose, the extent of gene induction among PmCYCD subgroups varied. The induction of PmCYCD1;2 by hormones depended on the presence of sucrose. PmCYCD3;1 was stimulated by NAA, and induction was strengthened when sugar and hormones were applied together. Taken together, our study demonstrates that PmCYCDs are functional in plant stem development and provides a basis for selecting members of the cyclin gene family as candidate genes for ornamental plant breeding.
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影响因子：
2.9
作者：
Sornay E;Dewitte W;Murray JA
通讯作者：
Murray JA
杨属中成束蛋白样阿拉伯半乳聚糖蛋白FLA6基因的反义表达抑制其同源基因的表达并改变转基因树中的茎生物力学和细胞壁组成
影响因子：
6.9
作者：
Wang H;Jiang C;Wang C;Yang Y;Yang L;Gao X;Zhang H
通讯作者：
Zhang H
DOI：
10.1105/tpc.7.1.85
发表时间：
1995
期刊：
The Plant cell
影响因子：
--
作者：
R. Soni;J. P. Carmichael;Z. Shah;J. Murray
通讯作者：
R. Soni;J. P. Carmichael;Z. Shah;J. Murray
影响因子：
7.4
作者：
D. Sorrell;M. Menges;J. M. Healy;Y. Deveaux;C. Amano;Ya Su;Hirofumi;Nakagami;A. Shinmyō;J. Doonan;M. Sekine;J. Murray
通讯作者：
D. Sorrell;M. Menges;J. M. Healy;Y. Deveaux;C. Amano;Ya Su;Hirofumi;Nakagami;A. Shinmyō;J. Doonan;M. Sekine;J. Murray
DOI：
10.1111/tpj.12957
发表时间：
2015-10
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Sornay E;Forzani C;Forero-Vargas M;Dewitte W;Murray JA
通讯作者：
Murray JA