uninflatable encodes a novel ectodermal apical surface protein required for tracheal inflation in Drosophila.
uninflatable encodes a novel ectodermal apical surface protein required for tracheal inflation in Drosophila.
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DOI：
10.1016/j.ydbio.2009.09.040
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发表时间：
2009-12-15
影响因子：
2.7
通讯作者：
Ward, Robert E.
中科院分区：
文献类型：
--
作者：
Zhang, Liang;Ward, Robert E.
果蝇的气管系统已被证明是研究分支管状器官发育的优秀模型系统。调节气管系统的模式和初始成熟的机制已经基本解决，但重要的问题仍然存在，即成熟的管子如何在胚胎发生结束时充气，以及气管系统如何响应发育中的幼虫的氧气需求而生长，幼虫的体积在四天内增加了近1000倍。在这里，我们描述了不可充气（uif）的克隆和表征，该基因编码在其胞外域中含有碳水化合物结合和细胞信号传导基序的大跨膜蛋白。 Uif 在昆虫物种中高度保守，但在脊椎动物物种中似乎没有真正的直系同源物。 uif 从第 5 阶段胚胎开始以合子方式表达，并且 Uif 蛋白定位于所有外胚层衍生上皮细胞的顶端质膜，尤其是在气管系统中。 uif突变动物在胚胎发生末期表现出气管膨胀缺陷，并且主要以幼虫的形式死亡。尽管气管形态和成熟在胚胎发生过程中表现正常，但突变幼虫的气管经常被压碎或扭曲。 uif突变体幼虫在气管生长和气管角质层蜕皮方面也表现出缺陷。
The tracheal system of Drosophila melanogaster has proven to be an excellent model system for studying the development of branched tubular organs. Mechanisms regulating the patterning and initial maturation of the tracheal system have been largely worked out, yet important questions remain regarding how the mature tubes inflate with air at the end of embryogenesis, and how the tracheal system grows in response to the oxygen needs of a developing larva that increases nearly 1000-fold in volume over a four day period. Here we describe the cloning and characterization of uninflatable (uif), a gene that encodes a large transmembrane protein containing carbohydrate binding and cell signaling motifs in its extracellular domain. Uif is highly conserved in insect species, but does not appear to have a true ortholog in vertebrate species. uif is expressed zygotically beginning in stage 5 embryos, and Uif protein localizes to the apical plasma membrane in all ectodermally derived epithelia, most notably in the tracheal system. uif mutant animals show defects in tracheal inflation at the end of embryogenesis, and die primarily as larvae. Tracheal tubes in mutant larvae are often crushed or twisted, although tracheal patterning and maturation appear normal during embryogenesis. uif mutants larvae also show defects in tracheal growth and molting of their tracheal cuticle.
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影响因子：
11.8
作者：
Behr, M;Riedel, D;Schuh, R
通讯作者：
Schuh, R
影响因子：
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作者：
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通讯作者：
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