Rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos.
Rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos.
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水稻条纹病毒通过改变胚胎中的发育基因表达来影响其载体后代的生存能力
DOI：
10.1038/srep07883
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发表时间：
2015-01-20
影响因子：
4.6
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Li S;Wang S;Wang X;Li X;Zi J;Ge S;Cheng Z;Zhou T;Ji Y;Deng J;Wong SM;Zhou Y
植物病毒可能影响其食草性载体的生存能力和发育过程。小褐飞虱（SBPH）是水稻条纹病毒（RSV）的主要媒介，是水稻条纹病的主要病原。在这里，我们通过病毒株和非病毒株的杂交实验报道了RSV对SBPH后代的影响。比较了不同杂交组合后代的寿命参数。毒毒亲本的f1子代的孵化率显著降低，毒率完全由毒毒亲本控制。为了更好地阐明潜在的生物学机制，研究了卵子的形态、病毒在卵子中的传播和分布以及胚胎发育基因的表达谱。结果表明，RSV在SBPH卵中复制和积累，导致部分卵发育迟缓或发育迟缓；此外，RSV仅能感染卵子而不能感染精子。从表达谱上看，13个发育基因在毒亲本卵中表达受到调控，其中两个重要调控基因Ls-DorsalandLs-CPO表达下调最为显著。一般情况下，RSV对SBPH有不利影响，不利于病毒种群的扩大。这一观点也得到了系统监测SBPH毒率的支持。
Plant viruses may affect the viability and development process of their herbivore vectors. Small brown planthopper (SBPH) is main vector of Rice stripe virus (RSV), which causes serious rice stripe disease. Here, we reported the effects of RSV on SBPH offspring by crossing experiments between viruliferous and non-viruliferous strains. The life parameters of offspring from different cross combinations were compared. The hatchability of F1progeny from viruliferous parents decreased significantly and viruliferous rate was completely controlled by viruliferous maternal parent. To better elucidate the underlying biological mechanisms, the morphology of eggs, viral propagation and distribution in the eggs and expression profile of embryonic development genes were investigated. The results indicated that RSV replicated and accumulated in SBPH eggs resulting in developmental stunt or delay of partial eggs; in addition, RSV was only able to infect ovum but not sperm. According to the expression profile, expression of 13 developmental genes was regulated in the eggs from viruliferous parents, in which two important regulatory genes (Ls-DorsalandLs-CPO) were most significantly down-regulated. In general, RSV exerts an adverse effect on SBPH, which is unfavourable for the expansion of viruliferous populations. The viewpoint is also supported by systematic monitoring of SBPH viruliferous rate.
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电镜研究水稻条纹病毒在小褐飞虱体内的亚细胞定位
影响因子：
4.8
作者：
Deng J;Li S;Hong J;Ji Y;Zhou Y
通讯作者：
Zhou Y
影响因子：
11.4
作者：
Brody, T
通讯作者：
Brody, T
DOI：
10.1073/pnas.0604484103
发表时间：
2006-08-22
影响因子：
11.1
作者：
Biemar, Frederic;Nix, David A.;Levine, Michael S.
通讯作者：
Levine, Michael S.
DOI：
10.1007/bf00868677
发表时间：
1980-01-01
期刊：
WILHELM ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
MINDRINOS, MN;PETRI, WH;MARGARITIS, LH
通讯作者：
MARGARITIS, LH
影响因子：
1.9
作者：
Keramaris, KE;Margaritis, LH;Tsiropoulos, GJ
通讯作者：
Tsiropoulos, GJ