Characterization of bacteriophage communities and CRISPR profiles from dental plaque.
Characterization of bacteriophage communities and CRISPR profiles from dental plaque.
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噬菌体群落和牙菌斑的CRISPR概况的表征。
DOI：
10.1186/1471-2180-14-175
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发表时间：
2014-06-30
期刊：
影响因子：
4.2
通讯作者：
Pride DT
中科院分区：
文献类型：
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作者：
Naidu M;Robles-Sikisaka R;Abeles SR;Boehm TK;Pride DT
牙菌斑是一个多样化和复杂的细菌群落的家园，但通常被认为是由相对较少的病毒居住。我们从4名健康人受试者的唾液和牙菌斑中取样，以确定牙菌斑是否由病毒群落构成，以及受试者或样品类型的病毒群落是否存在差异。我们发现，噬菌斑是由一个社区的噬菌体，其成员大多是特定的主题。有一个显着比例的病毒同源物之间共享的斑块和唾液病毒组在每个主题，这表明一些口腔病毒存在于这两个网站。我们还描述了口腔链球菌中的规则间隔短回文重复序列（CRISPR），因为它们的特征为口腔细菌可能能够抵抗的病毒提供了线索。虽然每个样本类型都有一些特定的CRISPR间隔区，但更多的是跨站点共享的，并且具有高度的受试者特异性。许多CRISPR间隔区与斑块中存在的病毒相匹配，这表明CRISPR基因座的进化可能对斑块来源的病毒具有特异性。我们对噬斑衍生病毒和CRISPR谱的受试者特异性的发现表明，人类病毒生态可能是高度个性化的。
Dental plaque is home to a diverse and complex community of bacteria, but has generally been believed to be inhabited by relatively few viruses. We sampled the saliva and dental plaque from 4 healthy human subjects to determine whether plaque was populated by viral communities, and whether there were differences in viral communities specific to subject or sample type. We found that the plaque was inhabited by a community of bacteriophage whose membership was mostly subject-specific. There was a significant proportion of viral homologues shared between plaque and salivary viromes within each subject, suggesting that some oral viruses were present in both sites. We also characterized Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in oral streptococci, as their profiles provide clues to the viruses that oral bacteria may be able to counteract. While there were some CRISPR spacers specific to each sample type, many more were shared across sites and were highly subject specific. Many CRISPR spacers matched viruses present in plaque, suggesting that the evolution of CRISPR loci may have been specific to plaque-derived viruses. Our findings of subject specificity to both plaque-derived viruses and CRISPR profiles suggest that human viral ecology may be highly personalized.
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DOI：
10.1073/pnas.1305923110
发表时间：
2013-06-25
影响因子：
11.1
作者：
Barr, Jeremy J.;Auro, Rita;Rohwer, Forest
通讯作者：
Rohwer, Forest
DOI：
10.1073/pnas.0308766101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Lepp, PW;Brinig, MM;Relman, DA
通讯作者：
Relman, DA
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
DOI：
10.1126/science.1159689
发表时间：
2008-08-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Brouns SJ;Jore MM;Lundgren M;Westra ER;Slijkhuis RJ;Snijders AP;Dickman MJ;Makarova KS;Koonin EV;van der Oost J
通讯作者：
van der Oost J
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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