Involvement of CD147 in alveolar bone remodeling and soft tissue degradation in experimental periodontitis
Involvement of CD147 in alveolar bone remodeling and soft tissue degradation in experimental periodontitis
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CD147参与实验性牙周炎牙槽骨重塑和软组织退化
DOI：
10.1111/jre.12435
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发表时间：
2017-08
影响因子：
3.5
通讯作者：
Cao Z.
中科院分区：
文献类型：
--
作者：
Yang D.;Liu R.;Liu L.;Liao H.;Wang C.;Cao Z.
背景和目标
本研究的目的是通过骨涎蛋白、骨钙素、骨桥蛋白和碱性磷酸酶(ALP)、牙槽骨骨小梁结构和破骨细胞数量的骨标记物，探讨分化簇147(CD147)在骨吸收和矿化中的可能作用。我们还研究了CD147在炎症和胶原分解中的作用。
材料和方法
雄性Wistar大鼠28只，随机分为4组，每组7只：健康组、牙周炎组、牙周炎+生理盐水组和牙周炎+抗CD147组。苏木精-伊红染色进行组织学评价。用显微计算机断层扫描评估牙槽骨丢失和骨小梁的微结构。用天狼星红染色评价胶原纤维的破坏情况。采用抗酒石酸酸性磷酸酶染色进行破骨细胞分析。免疫组织化学方法检测碱性磷酸酶、骨唾液蛋白、骨钙素和骨桥蛋白的表达。
结果
抗CD147治疗可明显抑制牙槽骨丢失和破骨细胞生成，改善骨体积/组织体积，增加牙槽骨骨小梁厚度。组织学染色结果显示，抗CD147抗体可明显减轻炎症反应，减少胶原纤维中降解区域的比例。CD147抗体可增强骨标志物(碱性磷酸酶、骨涎蛋白、骨钙素和骨桥蛋白)的表达。
结论
抗CD147作用的结果表明，CD147参与了牙槽骨矿化、破骨细胞的形成和骨小梁的微结构。抑制CD147可以增加成骨标志物的表达水平，增加牙槽骨的高度，抑制胶原纤维的降解。
BACKGROUND AND OBJECTIVE
The objective of this study was to investigate the possible roles of clusters of differentiation 147 (CD147) in bone resorption and mineralization through the bone markers of bone sialoprotein, osteocalcin, osteopontin and alkaline phosphatase (ALP), trabecular structure of alveolar bone and number of osteoclasts. We also investigated the effects of CD147 on inflammation and collagen breakdown.
MATERIAL AND METHODS
Twenty-eight male Wistar rats were randomly divided into four groups of seven animals each: healthy group, periodontitis group, periodontitis + saline group and periodontitis + anti-CD147 groups. Hematoxylin and eosin staining were used for histological assessment. Alveolar bone loss and trabecula microstructure were evaluated using micro-computed tomography. Collagen fiber breakdown was assessed via picrosirius red staining. Tartrate-resistant acid phosphatase staining was conducted for osteoclast analysis. The expressions of ALP, bone sialoprotein, osteocalcin and osteopontin were evaluated using immunohistochemistry.
RESULTS
Anti-CD147 treatment significantly inhibited alveolar bone loss and osteoclastogenesis, and improved the bone volume/tissue volume, and the trabecular thickness of alveolar bone. Histological staining revealed that anti-CD147 significantly reduced the infiltration of inflammation and limited the fractions of degraded areas in collagen fibers. The expression of bone markers (ALP, bone sialoprotein, osteocalcin and osteopontin) was enhanced by anti-CD147 treatment.
CONCLUSION
The results of the anti-CD147 treatment indicate that CD147 was involved in alveolar bone mineralization, osteoclastogenesis and trabecular microstructure. The inhibition of CD147 could increase the expression level of osteogenic markers, alveolar bone crest height and suppressed collagen fiber degradation.
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影响因子：
1.9
作者：
J. Xiang;Z. Cao;W. Dong;Chengzhang Li
通讯作者：
J. Xiang;Z. Cao;W. Dong;Chengzhang Li
影响因子：
2.5
作者：
U. Weidle;W. Scheuer;Daniela Eggle;S. Klostermann;H. Stockinger
通讯作者：
U. Weidle;W. Scheuer;Daniela Eggle;S. Klostermann;H. Stockinger
影响因子：
7.2
作者：
Katagiri, Takenobu;Watabe, Tetsuro
通讯作者：
Watabe, Tetsuro
影响因子：
18.6
作者：
Deas, David E.;Mealey, Brian L.
通讯作者：
Mealey, Brian L.
影响因子：
11.2
作者：
Steven M. Ellis;Kazuki Nabeshima;Chitra Biswas
通讯作者：
Steven M. Ellis;Kazuki Nabeshima;Chitra Biswas