Antioxidant activities of hydrolysates of Arca subcrenata prepared with three proteases.
Antioxidant activities of hydrolysates of Arca subcrenata prepared with three proteases.
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作者：
Song L;Li T;Yu R;Yan C;Ren S;Zhao Y
为了得到具有抗氧化活性的产品，研究了最佳水解酶和水解条件。采用中和酶、碱性酶和木瓜蛋白酶水解亚绿豆匀浆。采用正交设计优化水解条件，采用pH-stat法确定水解度。从对α、α-二苯基-β-苦基肼基（DPPH）自由基和过氧化氢的清除率等还原能力来看，alcalase水解物（AH）的抗氧化活性优于中和酶水解物（NH）和木瓜蛋白酶水解物（PH），其对DPPH自由基和过氧化氢的清除率EC50值分别为6.23 mg/ml和19.09 mg/ml。此外，与中和酶和木瓜蛋白酶水解产物相比，AH的分子量更低，肽氨基酸含量更高。因此，我们选择alcalase作为生产活性成分的最佳酶，因为它的水解产物具有最好的抗氧化活性，并且含有大量的潜在活性肽。
In order to get products with antioxidant activity from Arca subcrenata Lischke, the optimal hydrolase and hydrolysis conditions were investigated in the paper. Three proteases (neutrase, alcalase and papain) were applied to hydrolyze the homogenate of A. subcrenata. An orthogonal design was used to optimize hydrolysis conditions, and the pH-stat methods was used to determine the degree of hydrolysis. Viewed from the angle of reducing power, such as scavenging activities against α,α-diphenyl-β-picrylhydrazyl (DPPH) radical and hydrogen peroxide, the antioxidant activities of the alcalase hydrolysate (AH) were superior to neutrase hydrolysate (NH) and papain hydrolysate (PH), and its EC50 values in DPPH radical and hydrogen peroxide scavenging effect were 6.23 mg/ml and 19.09 mg/ml, respectively. Moreover, compared with products hydrolyzed by neutrase and papain, the molecular mass of AH was lower and its content of amino acid of peptides was higher. Therefore, alcalase was selected as the optimal enzyme to produce active ingredients since its hydrolysate exhibited the best antioxidant activity among them and possessed large amount of potential active peptides.
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