Growth and chemotaxis of nematodes reduced upon exposure to Third Fork Creek surface water.
Growth and chemotaxis of nematodes reduced upon exposure to Third Fork Creek surface water.
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DOI：
10.1186/s12302-021-00579-8
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发表时间：
2022
影响因子：
5.9
通讯作者：
Spence P
中科院分区：
文献类型：
--
作者：
Gerald C;Deshazo B;Patterson H;Spence P
第三叉河是流经北卡罗来纳州达勒姆市的一条历史受损的城市河流。秀丽隐杆线虫（秀丽隐杆线虫）是一种非寄生的、居住在土壤和水生的线虫，经常被用作生物和生态毒性模型。我们假设暴露在第三叉溪地表水中会抑制秀丽隐杆线虫的生长和趋化性。利用我们的环状实验模型，线虫被引诱穿过水样到达细菌食物源，从而观察趋化性。记录细菌食物源和有水源的培养皿中间3天的线虫总数。我们的研究结果表明，与对照组相比，暴露于第三叉溪水样的线虫在第三天的趋化性和生长减少（p值< 0.05）。这些探索性数据为位于历史悠久的黑人大学附近的第三叉溪的质量提供了有意义的见解。需要进一步的研究来阐明水污染物的浓度及其对人类健康的影响。这项研究的相关性在于秀丽隐杆线虫模型，该模型已被用于大量人类疾病和暴露研究，但可以用作水质损害的环境指标。
Third Fork Creek is a historically impaired urban stream that flows through the city of Durham, North Carolina. Caenorhabditis elegans (C. elegans) are non-parasitic, soil and aquatic dwelling nematodes that have been used frequently as a biological and ecotoxicity model. We hypothesize that exposure to Third Fork Creek surface water will inhibit the growth and chemotaxis of C. elegans. Using our ring assay model, nematodes were enticed to cross the water samples to reach a bacterial food source which allowed observation of chemotaxis. The total number of nematodes found in the bacterial food source and the middle of the plate with the water source was recorded for 3 days. Our findings suggest a reduction in chemotaxis and growth on day three in nematodes exposed to Third Fork Creek water samples when compared to the control (p value < 0.05). These exploratory data provide meaningful insight to the quality of Third Fork Creek located near a Historically Black University. Further studies are necessary to elucidate the concentrations of the water contaminants and implications for human health. The relevance of this study lies within the model C. elegans that has been used in a plethora of human diseases and exposure research but can be utilized as an environmental indicator of water quality impairment.
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影响因子：
6.8
作者：
Hoess, S.;Jaensch, S.;Roembke, J.
通讯作者：
Roembke, J.
影响因子：
4.1
作者：
Höss, S;Haitzer, M;Steinberg, CEW
通讯作者：
Steinberg, CEW
DOI：
10.1093/toxsci/kfn121
发表时间：
2008-11
期刊：
Toxicological sciences : an official journal of the Society of Toxicology
影响因子：
--
作者：
Leung MC;Williams PL;Benedetto A;Au C;Helmcke KJ;Aschner M;Meyer JN
通讯作者：
Meyer JN
影响因子：
3.8
作者：
Min, Hyemin;Youn, Esther;Shim, Yhong-Hee
通讯作者：
Shim, Yhong-Hee
DOI：
10.1126/sageke.2005.40.pe30
发表时间：
2005-10-05
期刊：
Science of aging knowledge environment : SAGE KE
影响因子：
--
作者：
Caswell-Chen, E P;Chen, J;Carey, J R
通讯作者：
Carey, J R