Humans have antibodies against a plant virus: evidence from tobacco mosaic virus.
Humans have antibodies against a plant virus: evidence from tobacco mosaic virus.
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DOI：
10.1371/journal.pone.0060621
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Friedland RP
中科院分区：
文献类型：
--
作者：
Liu R;Vaishnav RA;Roberts AM;Friedland RP
烟草花叶病毒（Tobacco mosaic virus，TMV）是一种广泛分布的植物病原体，存在于烟草（包括卷烟和无烟烟草）以及许多其他植物中。植物病毒不会在人类或其他哺乳动物中复制或引起感染。这项研究的目的是确定暴露于烟草制品是否会诱导人类对TMV的免疫反应。使用夹心ELISA法，我们检测血清中的抗TMV抗体（IgG，IgG 1，IgG 3，IgG 4，伊加和IgM）在所有的研究对象（20名健康吸烟者，20名无烟烟草使用者，和20名非吸烟者）。吸烟者血清抗TMV IgG抗体水平高于非吸烟者，而无烟烟草使用者血清抗TMV伊加水平低于吸烟者和非吸烟者。利用生物信息学，我们还发现人蛋白TOMM40L（线粒体外膜40同源物样移位酶）含有与TMV外壳蛋白的六个连续氨基酸的强同源性，并且TOMM40L肽显示出与抗TMV抗体的交叉反应性。吸烟或其他烟草制品的人患帕金森病的风险较低，但这种情况发生的机制尚不清楚。我们的研究结果表明TMV和人TOMM40 L之间的分子模拟提出了一个问题，即TMV是否在吸烟者中对帕金森病的发展有潜在的作用。植物病毒与人类疾病之间的分子模拟机制有待进一步研究。
Tobacco mosaic virus (TMV), a widespread plant pathogen, is found in tobacco (including cigarettes and smokeless tobacco) as well as in many other plants. Plant viruses do not replicate or cause infection in humans or other mammals. This study was done to determine whether exposure to tobacco products induces an immune response to TMV in humans. Using a sandwich ELISA assay, we detected serum anti-TMV antibodies (IgG, IgG1, IgG3, IgG4, IgA, and IgM) in all subjects enrolled in the study (20 healthy smokers, 20 smokeless-tobacco users, and 20 non-smokers). Smokers had a higher level of serum anti-TMV IgG antibodies than non-smokers, while the serum level of anti-TMV IgA from smokeless tobacco users was lower than smokers and non-smokers. Using bioinformatics, we also found that the human protein TOMM40L (an outer mitochondrial membrane 40 homolog – like translocase) contains a strong homology of six contiguous amino acids to the TMV coat protein, and TOMM40L peptide exhibited cross-reactivity with anti-TMV antibodies. People who smoke cigarettes or other tobacco products experience a lower risk of developing Parkinson’s disease, but the mechanism by which this occurs is unclear. Our results showing molecular mimicry between TMV and human TOMM40L raise the question as to whether TMV has a potential role in smokers against Parkinson’s disease development. The potential mechanisms of molecular mimicry between plant viruses and human disease should be further explored.
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DOI：
10.1378/chest.54.1.42
发表时间：
1968-01-01
期刊：
DISEASES OF THE CHEST
影响因子：
--
作者：
CARPENTER, CM;LECLAIR, RA
通讯作者：
LECLAIR, RA
影响因子：
1.7
作者：
Li, Wen-Wei;Yang, Ru;Cai, Ding-Fang
通讯作者：
Cai, Ding-Fang
影响因子：
--
作者：
EMRE, M;DEDECKER, C
通讯作者：
DEDECKER, C
影响因子：
4.1
作者：
Beraud, Dawn;Maguire-Zeiss, Kathleen A
通讯作者：
Maguire-Zeiss, Kathleen A
影响因子：
3.7
作者：
Colson P;Richet H;Desnues C;Balique F;Moal V;Grob JJ;Berbis P;Lecoq H;Harlé JR;Berland Y;Raoult D
通讯作者：
Raoult D