Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood.
Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood.
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X射线照射对回收血液中肝癌细胞和红细胞的影响。
DOI：
10.1038/s41598-017-08405-z
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发表时间：
2017-08-11
影响因子：
4.6
通讯作者：
Yan M
中科院分区：
文献类型：
--
作者：
Zhang FJ;Yang JT;Tang LH;Wang WN;Sun K;Ming Y;Muhammad KG;Zheng YF;Yan M
术中血液回收的广泛临床接受度及其在癌症手术中的应用仍然存在争议。到目前为止，还没有一种方法可以安全地消除癌细胞，同时保留红细胞。在这里，我们研究了一定剂量的直线加速器照射产生的X射线是否可以杀死肝癌细胞，同时保留红细胞。将HepG 2、SK-Hep 1或Huh 7细胞混合到从健康志愿者获得的红细胞等分试样中。将混合细胞分别给予30戈伊和50戈伊X射线照射后，通过MTT法、平板集落形成法、5-乙炔基-2 ′-脱氧尿苷掺入法和免疫功能低下小鼠皮下移植瘤模型，检测肿瘤细胞的存活率、克隆形成能力、DNA合成和致瘤性。分别于照射后0、12、24、48、72 h检测红细胞ATP、2，3-DPG、游离Hb、渗透脆性、血气指标及红细胞形态学变化。30戈伊X射线照射能有效抑制HepG 2、SK-Hep 1和Huh 7细胞的活力、增殖和致瘤性，而不明显损害红细胞的携氧能力、膜完整性和形态。结果提示，30戈伊X线照射对肝癌细胞的清除和红细胞的保存是安全的。
The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accelerator irradiation at a certain dose can kill hepatocarcinoma cells while preserving erythrocytes. HepG2, SK-Hep1 or Huh7 cells were mixed into the aliquots of erythrocytes obtained from healthy volunteers. After the mixed cells were exposed to 30 Gy and 50 Gy X-rays irradiation, the viability, clonogenicity, DNA synthesis and tumorigenicity of the tumor cells were determined by the MTT assay, plate colony formation, 5-ethynyl-2′-deoxyuridine incorporation, and subcutaneous xenograft implantation into immunocompromised mice. The ATP, 2,3-DPG, free Hb, osmotic fragility, blood gas variables in erythrocytes and morphology of erythrocytes at 0 h, 12 h, 24 h, 48 h, 72 h after irradiation were analyzed. X-ray irradiation at 30 Gy effectively inhibited the viability, proliferation, and tumorigenicity of HepG2, SK-Hep1 and Huh7 cells without noticeably damaging the ability of oxygen-carrying, membrane integrity and morphology of erythrocytes. Theses results suggest that X-ray at 30 Gy irradiation might be safe to eliminate hepatocarcinoma cells while preserving erythrocytes in salvaged blood.
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影响因子：
4.6
作者：
Gwak, MS;Lee, KW;Lee, SK
通讯作者：
Lee, SK
同时检索参与介质的温度相关吸收系数和电导率
影响因子：
4.6
作者：
Ren, Yatao;Qi, Hong;Tan, Heping
通讯作者：
Tan, Heping
影响因子：
2.9
作者：
Karon BS;Hoyer JD;Stubbs JR;Thomas DD
通讯作者：
Thomas DD
DOI：
10.1186/1756-9966-30-7
发表时间：
2011-01-12
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Pinnarò P;Soriani A;D'Alessio D;Giordano C;Foddai ML;Pinzi V;Strigari L
通讯作者：
Strigari L
DOI：
10.1073/pnas.0910785107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Park, YongKeun;Best, Catherine A.;Feld, Michael S.
通讯作者：
Feld, Michael S.