Using self-assembled monolayers to model the extracellular matrix.
Using self-assembled monolayers to model the extracellular matrix.
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DOI：
10.1016/j.actbio.2009.01.016
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发表时间：
2009-03
影响因子：
9.7
通讯作者：
Mrksich, Milan
中科院分区：
文献类型：
--
作者：
Mrksich, Milan
细胞外基质是大蛋白质和糖胺聚糖的不溶性聚集体，构成组织中细胞的微环境。该基质展示了许多配体，这些配体与细胞表面受体相互作用，介导细胞的附着和扩散并调节信号传导过程。细胞-基质相互作用和下游信号传导过程的研究通常采用具有蛋白质吸附层的底物，并且由于难以控制固定化蛋白质的结构和活性而具有挑战性。人们付出了巨大的努力来开发模型基底，该模型基底以规定的密度、方向和环境呈现粘附配体。在这些方法中，金上自组装的链烷硫醇单层可以对表面分子结构进行高水平的控制，非常适合细胞粘附的研究。这篇综述描述了单层膜在细胞生物学中的应用的设计和使用，包括使用单层膜来评估肽和蛋白质配体在细胞-基质相互作用中的作用，单层膜上配体图案化方法的开发以及在细胞生物学中的应用，动态单层膜的开发，该动态单层膜可以切换呈递给贴壁细胞的配体的活性，以及细胞与其基质之间相互作用的重新布线。这些例子说明了单层细胞在细胞生物学应用中固有的灵活性。
The extracellular matrix is an insoluble aggregate of large proteins and glycosoaminoglycans that comprises the microenvironment of cells in tissue. The matrix displays a host of ligands that interact with cell-surface receptors to mediate the attachment and spreading of cells and regulate signaling processes. Studies of cell–matrix interactions and downstream signaling processes commonly employ substrates having an adsorbed layer of protein and are challenging because of the difficulty in controlling the structure and activity of the immobilized protein. Significant effort has been directed towards the development of model substrates that present adhesion ligands in defined densities, orientations and environments. Among these approaches, self-assembled monolayers of alkanethiolates on gold offer a high level of control over the molecular structure of the surface and are well-suited to studies of cell adhesion. This review describes the design and use of monolayers for applications in cell biology, including the use of monolayers to evaluate the roles of peptide and protein ligands in cell–matrix interactions, the development of methods to pattern ligands on monolayers and applications to cell biology, the development of dynamic monolayers that can switch the activities of ligands presented to an adherent cell, and the rewiring of interactions between a cell and its substrate. These examples illustrate the flexibility inherent to monolayers for applications in cell biology.
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影响因子：
15
作者：
Hodneland, CD;Mrksich, M
通讯作者：
Mrksich, M
DOI：
10.1073/pnas.072685299
发表时间：
2002-04-16
影响因子：
11.1
作者：
Hodneland, CD;Lee, YS;Mrksich, M
通讯作者：
Mrksich, M
影响因子：
3.5
作者：
MARDON, HJ;GRANT, KE
通讯作者：
GRANT, KE
影响因子：
5
作者：
Chen, CS;Jiang, XY;Whitesides, GM
通讯作者：
Whitesides, GM
DOI：
10.1073/pnas.0504349103
发表时间：
2006-02-14
影响因子：
11.1
作者：
Collier, JH;Mrksich, M
通讯作者：
Mrksich, M