Measurement of Circulating Phospholipid Fatty Acids: Association between Relative Weight Percentage and Absolute Concentrations.
Measurement of Circulating Phospholipid Fatty Acids: Association between Relative Weight Percentage and Absolute Concentrations.
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DOI：
10.1080/07315724.2015.1116417
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发表时间：
2016-09
影响因子：
3.5
通讯作者：
Neuhouser ML
中科院分区：
文献类型：
--
作者：
Song X;Schenk JM;Diep P;Murphy RA;Harris TB;Eiriksdottir G;Gudnason V;Casper C;Lampe JW;Neuhouser ML
大多数关于循环磷脂脂肪酸（PLFA）和疾病风险的流行病学研究都使用每种脂肪酸的相对浓度（占总量的百分比）作为暴露的量度。使用相对浓度，所有脂肪酸的总和为100%，因此单个脂肪酸的值不是独立的。这导致了辩论，沿着的建议，使用绝对浓度的脂肪酸。我们的目的是检查个体循环PLFA的相对（重量百分比）和绝对（mg/L）浓度之间的关系。41循环PLFA的相对和绝对浓度进行了测定，通过气相色谱法在三个不同的人群的样品。每种脂肪酸的相对和绝对浓度之间的相关性用于测量一致性。计算未校正的相关性和校正总胆固醇的绝对PLFA浓度的相关性。在所有三项研究中，大多数PLFA的相对和绝对浓度之间的未校正相关性以及调整总胆固醇绝对PLFA浓度的相关性都很高。在这3项研究中，41项分析的PLFA中有28项的未校正相关性>0.6，39项的校正相关性>0.6。相对浓度与绝对浓度的选择可能不会影响个体PLFA与疾病结局之间相关性研究中大多数循环PLFA结果的解释，特别是如果模型中包含反映总脂质（如总循环胆固醇）的协变量。然而，对于脂肪酸，例如16：0（棕榈酸），两个指标之间的相关性较低，使用相对浓度与绝对浓度可能导致关于其与结果的关联的不同推断。由于两种浓度可以同时从同一实验室测定中获得，因此需要使用两种指标来更好地了解PLFA-疾病关系。
Most epidemiologic studies of circulating phospholipid fatty acids (PLFAs) and disease risk have used the relative concentration (percentage of total) of each fatty acid as the measure of exposure. Using relative concentrations, the total of all fatty acids are summed to 100% and thus the values of individual fatty acid are not independent. This has led to debate, along with the suggestion to use absolute concentrations of fatty acids. We aimed to examine the relationship between relative (weight percentage) and absolute (mg/L) concentrations of individual circulating PLFAs. Relative and absolute concentrations of 41 circulating PLFAs were measured by gas chromatography in samples from three diverse populations. Correlations between the relative and absolute concentrations for each fatty acid were used to measure agreement. Unadjusted correlations and correlations adjusting absolute PLFA concentrations for total cholesterol were calculated. Unadjusted correlations between relative and absolute concentrations, as well as correlations adjusting absolute PLFA concentrations for total cholesterol, were high for most PLFAs in all three studies. Across the 3 studies, 28 of the 41 analyzed PLFAs had unadjusted correlations >0.6 and 39 had adjusted correlations >0.6. Choice of relative vs. absolute concentration may not affect interpretation of results for most circulating PLFAs in studies of association between individual PLFAs and disease outcomes, especially if a covariate reflecting total lipids, such as total circulating cholesterol, is included in the model. However, for fatty acids, such as 16:0 (palmitic acid), with low correlation between the two metrics, using relative vs. absolute concentration may lead to different inferences regarding their association with the outcome. Since both concentrations could be obtained simultaneously from the same laboratory assay, use of both metrics is warranted to better understand PLFA-disease relationships.
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影响因子：
5.9
作者：
Schwingshackl L;Hoffmann G
通讯作者：
Hoffmann G
影响因子：
7.1
作者：
Lindberg, Morten;Saltvedt, Ingvild;Bjerve, Kristian S.
通讯作者：
Bjerve, Kristian S.
将脂肪酸分配到血脂级分中的差异：Linoleate，Oleate和棕榈酸酯的比较。
DOI：
10.1152/ajpendo.90730.2008
发表时间：
2009-01
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
Hodson L;McQuaid SE;Karpe F;Frayn KN;Fielding BA
通讯作者：
Fielding BA
影响因子：
7.1
作者：
DOUGHERTY, RM;GALLI, C;IACONO, JM
通讯作者：
IACONO, JM
影响因子：
10.3
作者：
Brasky, Theodore M.;Darke, Amy K.;Kristal, Alan R.
通讯作者：
Kristal, Alan R.