Differences in partitioning of meal fatty acids into blood lipid fractions: a comparison of linoleate, oleate, and palmitate.
Differences in partitioning of meal fatty acids into blood lipid fractions: a comparison of linoleate, oleate, and palmitate.
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将脂肪酸分配到血脂级分中的差异：Linoleate，Oleate和棕榈酸酯的比较。
DOI：
10.1152/ajpendo.90730.2008
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Fielding BA
中科院分区：
文献类型：
--
作者：
Hodson L;McQuaid SE;Karpe F;Frayn KN;Fielding BA
人们对膳食脂肪的健康影响很感兴趣，但很少有研究全面比较体内特定脂肪酸的急性代谢命运。我们假设，不同类别的脂肪酸将在代谢途径中被重新分配，并且这将在24小时内变得明显。我们在12名健康受试者的测试早餐中使用等量的[U-13 C]亚油酸酯、[U-13 C]油酸酯和[U-13 C]棕榈酸酯追踪脂肪酸的命运。血浆乳糜微粒-三酰甘油（TG）中示踪剂浓度有差异的趋势（油酸酯>棕榈酸酯>亚油酸酯）。该模式在血浆非酯化脂肪酸（NEFA）和极低密度脂蛋白（VLDL）-TG中保持不变（对于NEFA和VLDL-TG，[U-13 C]油酸酯与[U-13 C]棕榈酸酯和[U-13 C]亚油酸酯分别为P ≤ 0. 01和P ≤ 0. 02）。在血浆胆固醇酯和磷脂（PL）中，[U-13 C]亚油酸酯显著多于其他两种示踪剂。使用同位素富集的值在不同的脂质馏分与测试餐相比，我们计算了膳食脂肪酸的贡献，各自的馏分。24小时后，10%的血浆PL-亚油酸来自早餐测试餐。这显著大于油酸和棕榈酸（均为3 ± 0.3%; P < 0.05）。红细胞PL脂肪酸也是如此。亚油酸从单餐到血液PL组分的显着快速掺入可能具有功能性后果，如维持膜流动性，并可以解释为什么亚油酸是一个有用的生物标志物的饮食摄入量。
There has been much interest in the health effects of dietary fat, but few studies have comprehensively compared the acute metabolic fate of specific fatty acids in vivo. We hypothesized that different classes of fatty acids would be variably partitioned in metabolic pathways and that this would become evident over 24 h. We traced the fate of fatty acids using equal amounts of [U-13C]linoleate, [U-13C]oleate, and [U-13C]palmitate given in a test breakfast meal in 12 healthy subjects. There was a tendency for differences in the concentrations of the tracers in plasma chylomicron-triacylglycerol (TG) (oleate > palmitate > linoleate). This pattern remained in plasma nonesterified fatty acid (NEFA) and very low-density lipoprotein (VLDL)-TG (P ≤ 0.01 and P ≤ 0.02 for [U-13C]oleate vs. both [U-13C]palmitate and [U-13C]linoleate for NEFA and VLDL-TG, respectively). There was significantly more [U-13C]linoleate than the other two tracers in plasma cholesteryl ester and phospholipid (PL). Using the values for isotopic enrichment in the different lipid fractions compared with the test meal, we calculated the contribution of meal fatty acids to the respective fractions. At 24 h, 10% of plasma PL-linoleate originated from the breakfast test meal. This was significantly greater than for oleate and palmitate (both 3 ± 0.3%; P < 0.05). This pattern was also true for erythrocyte PL fatty acids. The marked rapid incorporation of linoleate from a single meal into blood PL fractions may have functional consequences such as maintenance of membrane fluidity and may explain why linoleate is a useful biomarker of dietary intake.
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